Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cohesins02:20

Cohesins

5.8K
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...
5.8K
Cohesins02:20

Cohesins

2.4K
2.4K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

9.9K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.9K
Nucleosome Remodeling02:54

Nucleosome Remodeling

11.5K
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
11.5K
Chromatin Packaging02:21

Chromatin Packaging

22.8K
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order...
22.8K
Chromatin Packaging02:21

Chromatin Packaging

10.1K
10.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

LoopID reveals condensate-mediated enhancer-promoter interaction regulates cell fate.

Life medicine·2026
Same author

Decoding and engineering 3D chromatin interactions governing pluripotent and totipotent cell states.

Current opinion in genetics & development·2026
Same author

RYBP regulates selective genomic binding of TrxG and PcG components in embryonic stem cell fate control.

The EMBO journal·2026
Same author

Nuclear N-glycosylation maintains H3K9me3 heterochromatin and genomic stability.

Nature cell biology·2026
Same author

iPSC-derived breast cancer models: advancing the study of <i>BRCA1</i>-driven tumorigenesis.

Life medicine·2026
Same author

Phase separation of DUX family proteins drives totipotent-like state via 3D genome reorganization and retrotransposon activation.

Protein & cell·2026

Related Experiment Video

Updated: Mar 14, 2026

In-Nucleus Hi-C in Drosophila Cells
11:58

In-Nucleus Hi-C in Drosophila Cells

Published on: September 15, 2021

4.8K

A first-principles quantitative framework for how cohesin regulators shape chromatin loop extrusion.

Zibin Huang1, Xinyi Liu1, Junjun Ding1

  • 1Center for Stem Cell Biology and Tissue Engineering, Key Laboratory for Stem Cells and Tissue Engineering, Ministry of Education, Department of Histology and Embryology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong, China.

Cell Genomics
|March 12, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed a new model for loop extrusion, a key process in genome organization. This "bursty extrusion" framework explains how cohesin-associated factors quantitatively regulate DNA looping and 3D genome architecture.

More Related Videos

CRISPR-Mediated Reorganization of Chromatin Loop Structure
09:20

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Published on: September 14, 2018

13.2K
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.8K

Related Experiment Videos

Last Updated: Mar 14, 2026

In-Nucleus Hi-C in Drosophila Cells
11:58

In-Nucleus Hi-C in Drosophila Cells

Published on: September 15, 2021

4.8K
CRISPR-Mediated Reorganization of Chromatin Loop Structure
09:20

CRISPR-Mediated Reorganization of Chromatin Loop Structure

Published on: September 14, 2018

13.2K
HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

8.8K

Area of Science:

  • Genomics
  • Molecular Biology
  • Biophysics

Background:

  • Loop extrusion is a fundamental mechanism for organizing the 3D genome.
  • Cohesin-associated factors play crucial roles in regulating this process.
  • Quantitative models are needed to understand the complex dynamics of genome architecture.

Purpose of the Study:

  • To develop a first-principles framework for quantitatively modeling loop extrusion.
  • To investigate the role of multiple cohesin-associated factors in regulating loop extrusion.
  • To predict the impact of regulatory factors on motor kinetics and chromatin organization.

Main Methods:

  • Development of a first-principles biophysical model.
  • Quantitative analysis of loop extrusion dynamics.
  • Simulation of chromatin contact patterns and chromosome morphology.

Main Results:

  • Introduction of the "bursty extrusion" model.
  • Demonstration of quantitative regulation by multiple cohesin-associated factors.
  • Prediction of regulator-dependent changes in motor kinetics and 3D genome structure across scales.

Conclusions:

  • The "bursty extrusion" framework provides a mechanistically grounded basis for quantitative modeling of 3D genome architecture.
  • The model predicts how cohesin-associated factors influence genome organization.
  • This work advances our understanding of the biophysical principles governing genome folding.