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Related Experiment Video

Updated: Mar 14, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol

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Published on: October 24, 2016

Reprogramming Carbon Partition for Salidroside Overproduction in Saccharomyces cerevisiae.

Jian Li^1,2,3, Honghao Li^1,2,3, Xiaoran Dai^1,2,3

¹Frontiers Science Center for Synthetic Biology (Ministry of Education), School of Synthetic Biology and Biomanufacturing, Tianjin University, Tianjin 300072, China.

ACS Synthetic Biology

|March 13, 2026

Summary

This summary is machine-generated.

Microbial production of salidroside was enhanced by engineering central metabolism and cell wall synthesis. This strategy overcame UDP-glucose competition, achieving a record 40.46 g/L titer for this valuable glycoside.

Keywords:

UDP-glucose pathway cell wall engineering cofactor engineering metabolic engineering salidroside

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Area of Science:

Biotechnology and Synthetic Biology
Metabolic Engineering
Natural Product Biosynthesis

Background:

Salidroside is a valuable plant-derived glycoside with significant nutraceutical and pharmaceutical potential.
Microbial production of salidroside is limited by the competition for UDP-glucose (UDPG) between cell wall synthesis and salidroside formation.

Purpose of the Study:

To engineer a high-yielding microbial cell factory for enhanced salidroside production.
To overcome the bottleneck of UDP-glucose (UDPG) competition in glycoside synthesis.

Main Methods:

Rewired central metabolism using thiamine diphosphate (ThDP) regeneration for increased tyrosol precursor production.
Introduced a glycosyltransferase (RrU8GT33) for tyrosol to salidroside conversion.
Enhanced UDP-glucose (UDPG) availability via UGP1 and PGM1 overexpression.
Implemented cell wall engineering by regulating FKS1 expression to redirect carbon flux.

Main Results:

Achieved a record-breaking salidroside titer of 40.46 g/L in bioreactor fermentation.
Demonstrated a productivity of 0.24 g/(L h) and a yield of 0.27 g/g glucose.
Successfully decoupled growth pressure from salidroside synthesis demand through integrated engineering strategies.

Conclusions:

Cofactor and cell wall engineering are effective strategies for optimizing microbial glycoside production.
Developed a scalable approach for the industrial manufacturing of high-value natural glycosides like salidroside.