Related Experiment Video
Updated: Mar 14, 2026
The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes
Published on: April 10, 2015
Enantiomers of Ruthenium(II) Complex [Ru(bpy)2(qip)]2+ Exhibit Highly Chiral-Selective Effects on the Stabilization
Xinxin Ling1,2, Lifeng Tan1,2
1Key Lab of Environment-Friendly Chemistry and Application in Ministry of Education, Xiangtan University, China.
Abstract:
Two chiral Ru(II) complexes bearing a quinoline ring, Λ-[Ru(bpy)2(qip)]2+ (Λ-1) and Δ-[Ru(bpy)2(qip)]2+ (Δ-1, where bpy = 2,2'-bipyridine and qip = 2-(quinolin-3-yl)-1H-imidazo[4,5-f][1,10]phenanthroline), were prepared. Their interactions with triplex RNA were systematically investigated using ultraviolet-visible (UV-Vis) spectroscopy, luminescence spectroscopy, nucleic acid thermal denaturation experiments, and circular dichroism (CD) spectroscopy. The data from UV-Vis titrations support intercalation as the binding mode for both enantiomers with triplex RNA. Further quantitative analysis revealed that the complexes exhibited significant chiral selectivity, with Δ-1 demonstrating a stronger binding affinity for triplex RNA than Λ-1. Luminescence titration experiments further supported the binding of the complexes via intercalation. CD analysis demonstrated that both Λ-1 and Δ-1 could significantly induce conformational changes in the triplex RNA, and strong intermolecular interactions between the complexes and triplex RNA were observed, further suggesting a potential intercalative binding mode. The thermal denaturation results revealed that the third strand was selectively and markedly stabilized. The stabilization of the third strand in the triplex RNA was significantly enhanced by both enantiomers, while exerting negligible effects on the template duplex. The chiral ruthenium(II) complex developed in this study exhibits specific stabilization of triplex RNA, providing an effective strategy for stabilizing triplex RNA structures.
More Related Videos
07:12Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor
Published on: October 26, 2017
09:04Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
Published on: September 21, 2017
Related Concept Videos
01:53RNA Stability
02:30Chirality at Nitrogen, Phosphorus, and Sulfur
A consequence of chirality is the need for enantiomeric resolution. While this is theoretically possible for all...
02:36Transfer RNA Synthesis
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
01:19RNA Structure
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
01:23RNA Structure
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
01:13Olefin Metathesis Polymerization: Overview
Ruthenium-based Grubbs catalyst is the most commonly used catalyst for olefin metathesis polymerization. Grubbs catalyst consists of a...