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Effect of calcium dobesilate on liver regeneration in rats undergoing partial hepatectomy
Gizem Fırtına1, Hamdi Taner Turgut2
1Department of Gastroenterology Surgery, Trabzon Training and Research Hospital, Health Sciences University, Trabzon-Türkiye.
Background:
To date, no study has evaluated the effects of calcium dobesilate on regenerative capacity after partial hepatectomy. Within the scope of this research, we aimed to elucidate the effects of calcium dobesilate (CD) on liver regeneration capacity and antioxidant pathways after partial hepatectomy.
Methods:
Thirty-six Sprague Dawley male rats weighing between 250-350 grams were used in the study. All animals underwent partial hepatectomy. The rats were randomly divided into four groups, each consisting of nine rats, as control groups (Groups 1 and 2) and study groups (Groups 3 and 4). Regeneration rate, histopathological parameters, immunohistochemical examination, and the apoptotic index (AI) were measured.
Results:
Tissue superoxide dismutase (SOD) levels were statistically significantly higher in the calcium dobesilate study groups compared to controls (p=0.03). Malondialdehyde (MDA) levels were statistically significantly higher in the study groups than in the control groups on both the second and seventh days (p=0.001). The regeneration rate (RR) was higher in the study group compared to the control group on the second day, and this difference was statistically significant (p<0.001). RR was also significantly higher in the study group on the second day compared to the seventh day (p<0.001). According to the Suzuki Scoring System, vacuolization and necrosis were not observed in the study groups (p<0.001 vs. p=0.034, respectively). The apoptotic index was significantly higher in the control groups compared to the study groups (p<0.001), and AI was statistically significantly lower on the seventh day (p=0.006). Ki-67 expression was statistically significantly higher in the groups receiving CD treatment on both the second and seventh days. In the control groups, Ki-67 expression was statistically significantly higher on the seventh day compared to the second day (p=0.006).
Conclusion:
This research indicated the effects of calcium dobesilate on improving oxidative damage and liver regeneration in rats undergoing partial hepatectomy. The results of the present study showed that (preoperative-postoperative) CD improves oxidative stress and increases liver regeneration capacity after partial hepatectomy.
