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Transcriptomic identification and developmental mapping of nrg3b expression in zebrafish.
Elham Tarahomi1, Nuray Söğünmez Erdoğan2
1Department of Computational Applied Science and Engineering, School of Graduate Studies, Kadir Has University, İstanbul, Turkiye.
Turkish Journal of Biology = Turk Biyoloji Dergisi
|March 13, 2026
Summary
Zebrafish nrg3b expression increases during development, particularly in excitatory neurons, suggesting a role in synaptic organization. This study maps nrg3b
Area of Science:
- Neuroscience
- Developmental Biology
- Genetics
Background:
- Neuregulin 3 (NRG3) is crucial for mammalian neuronal circuit formation.
- The expression pattern of its zebrafish ortholog, nrg3b, is not well-defined.
- Previous transcriptomic data suggested nrg3b dysregulation in zebrafish sco-spondin mutants.
Purpose of the Study:
- To characterize the temporal and spatial expression of nrg3b in zebrafish.
- To investigate the role of nrg3b during embryonic and adult neurodevelopment.
Main Methods:
- Analysis of RNA-seq data from zebrafish sco-spondin mutants.
- Quantitative reverse transcription polymerase chain reaction (RT-qPCR) for temporal expression.
- Whole-mount in situ hybridization (WISH) for spatial expression and neuronal subtype analysis.
- In situ hybridization (ISH) on adult brain sections.
Main Results:
- nrg3b expression significantly increased at 3 and 5 days postfertilization (dpf).
- Strong nrg3b expression was observed in anterior brain and spinal cord at 5 dpf, overlapping with glutamatergic markers.
- Adult zebrafish brains showed nrg3b expression in telencephalic nuclei.
Conclusions:
- nrg3b expression is stage-dependent and enriched in excitatory neuronal regions during zebrafish development.
- Sustained nrg3b expression in the adult telencephalon suggests a role in synaptic organization.
- This study provides a basis for further research on the Nrg3b-ErbB4 signaling pathway in zebrafish.
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