Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fast label-free live imaging with FlowVision reveals key principles of cancer cell arrest on endothelial monolayers.

The EMBO journal·2026
Same author

PhotoFiTT: a quantitative framework for assessing phototoxicity in live-cell microscopy experiments.

Nature communications·2025
Same author

Regulation of cell dynamics by rapid integrin transport through the biosynthetic pathway.

The Journal of cell biology·2025
Same author

Roles of protein-protein interactions and monolayer mechanics in tricellulin localization to tricellular tight junctions.

Biology open·2025
Same author

Structural Repetition Detector for multi-scale quantitative mapping of molecular complexes through microscopy.

Nature communications·2025
Same author

Comparison of Automatic Segmentation and Preprocessing Approaches for Dynamic Total-Body 3D Pet Images with Different Pet Tracers.

Journal of imaging informatics in medicine·2025
Same journal

An open-source high-precision hive for long-term honeybee observation and research.

Biology open·2026
Same journal

Signaling through RXRb and its agonist, bexarotene, promotes neuron formation in Xenopus laevis embryos.

Biology open·2026
Same journal

Testing the limits: the impact of extreme functional hip width on the muscular demands of walking.

Biology open·2026
Same journal

Retraction: Assessment of seasonal, substrate and hormonal factors influencing the propagation and rooting of Camellia rubriflora's cuttings.

Biology open·2026
Same journal

Opsin gene evolution in aquatic, amphibious and terrestrial blennies (Blenniidae).

Biology open·2026
Same journal

Lipid droplet size profiling in yeast.

Biology open·2026
See all related articles

Related Experiment Video

Updated: Mar 14, 2026

Long-term Live Imaging Device for Improved Experimental Manipulation of Zebrafish Larvae
14:05

Long-term Live Imaging Device for Improved Experimental Manipulation of Zebrafish Larvae

Published on: October 27, 2017

9.3K

Customizable FDM-based zebrafish larva mold for live imaging.

Marcela Xiomara Rivera Pineda1,2,3, Jaakko Lehtimäki1,2,3, Guillaume Jacquemet1,2,3,4

  • 1Faculty of Science and Engineering, Cell Biology, Åbo Akademi University, 20520 Turku, Finland.

Biology Open
|March 13, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a 3D-printed tool for orienting zebrafish larvae for live imaging. The fused deposition modeling (FDM) method offers a cost-effective and reproducible solution for high-resolution imaging workflows.

Keywords:
3D printingFused deposition modelingLive imagingZebrafish larvae

More Related Videos

A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos
08:55

A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos

Published on: January 14, 2020

10.1K
Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae
07:40

Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae

Published on: December 8, 2017

10.4K

Related Experiment Videos

Last Updated: Mar 14, 2026

Long-term Live Imaging Device for Improved Experimental Manipulation of Zebrafish Larvae
14:05

Long-term Live Imaging Device for Improved Experimental Manipulation of Zebrafish Larvae

Published on: October 27, 2017

9.3K
A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos
08:55

A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos

Published on: January 14, 2020

10.1K
Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae
07:40

Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae

Published on: December 8, 2017

10.4K

Area of Science:

  • Developmental Biology
  • Bioimaging
  • Additive Manufacturing

Background:

  • Accurate orientation of zebrafish larvae is crucial for high-resolution live imaging.
  • Standard agarose mounting methods present challenges for reproducible larval positioning.
  • Previous 3D-printing methods often rely on stereolithography (SLA).

Purpose of the Study:

  • To develop an improved, cost-effective 3D-printed tool for consistent zebrafish larval orientation.
  • To enhance existing additive manufacturing techniques for zebrafish imaging applications.
  • To facilitate reliable, long-term live imaging of multiple zebrafish larvae.

Main Methods:

  • Utilizing fused deposition modeling (FDM) 3D printing to create larval orientation molds.
  • Applying a thin resin coating to enhance the surface smoothness and performance of the FDM prints.
  • Designing molds with consistent, larval-shaped wells for dorsoventral orientation.
  • Ensuring compatibility with standard glass-bottom imaging dishes and providing open-source design files for customization.

Main Results:

  • The FDM-printed molds provide consistent and reproducible dorsoventral orientation of zebrafish larvae.
  • The resin coating improves the surface quality and functionality of the 3D-printed orientation tools.
  • The low-cost, customizable molds are suitable for standard imaging setups.
  • The method enables reliable, long-term live imaging of multiple larvae.

Conclusions:

  • Fused deposition modeling (FDM) with resin enhancement offers a practical and affordable alternative for creating zebrafish larval orientation tools.
  • This approach streamlines live imaging workflows by improving larval positioning accuracy and reproducibility.
  • The study highlights the expanding utility of low-cost additive manufacturing in biological research and imaging.