Mirogabalin Eliminated Pain and Reduced Inflammation in Visceral Pain

Mirogabalin is a newly developed gabapentinoid drug. Several in vivo and clinical studies have demonstrated the potent analgesic effects of mirogabalin in neuropathic pain. This study aims to investigate the impact of mirogabalin on visceral pain and inflammation. Adult male Balb/c mice (20-25 g) were used in the study (n=7). Mirogabalin was administered intraperitoneally at 10, 20, and 40 mg/kg doses. Inflammatory visceral pain was induced by intraperitoneal administration of acetic acid. The number of writhing was observed after acetic acid administration, and the effective dose of mirogabalin was determined. In the second phase of the study, the effects of mirogabalin on locomotor activity and leukocyte infiltration into peritoneal tissue were examined. IL-6, GSH levels, and SOD activity were investigated biochemically. Statistical analyses were performed in the GraphPad Prism (v8.0.1) program. Mirogabalin was significantly antinociceptive at all three doses (p<0.001). Histopathologic examination showed that the effective dose of mirogabalin decreased leukocyte infiltration into the peritoneum. Mirogabalin did not affect total distance moved and mean speed in the open field test. There was no significant difference between the groups in terms of IL-6, GSH levels, and SOD activity. Our results demonstrated a significant antinociceptive effect of mirogabalin against visceral pain. In addition, anti-inflammatory effects were revealed by decreasing leukocyte infiltration. However, the fact that mirogabalin did not alter antioxidant systems and IL-6 levels suggests that other mechanisms are responsible for its anti-inflammatory effects. Key words Mirogabalin " Pain " Inflammation " Visceral " GABA.