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Draft genome sequence of CTX-M-5 variant-carrying Kluyvera ascorbata isolated from river water in Kumamoto, Japan

Takatoshi Yamamoto1, Chie Shitada2,3, Tatsuya Kawaguchi1

  • 1Department of Medical Technology, Faculty of Health Sciences, Kumamoto Health Science University, Kumamoto, Japan.

Microbiology Resource Announcements
|March 13, 2026
PubMed

Insights

The genus Kluyvera likely originated extended-spectrum beta-lactamase (ESBL) genes. Researchers sequenced the Kluyvera ascorbata genome from a CTX-M-5 variant isolate found in Japan.

Area of Science:

  • Microbiology
  • Genomics
  • Antimicrobial Resistance

Background:

  • The genus Kluyvera is considered the likely origin of blaCTX-M genes.
  • These genes encode prevalent extended-spectrum beta-lactamases (ESBLs), crucial in antimicrobial resistance.
  • ESBLs confer resistance to broad-spectrum beta-lactam antibiotics.

Purpose of the Study:

  • To perform whole-genome sequencing of a Kluyvera ascorbata isolate.
  • To analyze the genome of a strain producing a CTX-M-5 variant.
  • To contribute genomic data for understanding the evolution of ESBL genes.

Main Methods:

  • Draft genome sequencing of Kluyvera ascorbata strain KHSU-R5-7.
  • Isolate sourced from Shirakawa River, Kumamoto, Japan.
  • Bioinformatic analysis of the sequenced genome.

Main Results:

  • The draft genome sequence of Kluyvera ascorbata strain KHSU-R5-7 was successfully obtained.
  • The isolate was identified as a producer of a CTX-M-5 variant.
  • Genomic data provides insights into the genetic makeup of the Kluyvera genus.

Conclusions:

  • This study reports the genome sequence of a significant Kluyvera ascorbata isolate.
  • The findings support the role of Kluyvera as a reservoir for blaCTX-M genes.
  • Further genomic studies are needed to fully elucidate the dissemination of ESBLs.
Keywords:
CTX-MESBLKluyvera

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