Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

2.2K
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
2.2K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.6K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.6K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

831
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
831
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

1.8K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A dual colorimetric and fluorescent sensor for non-enzymatic glucose detection via catalytic reaction with o-phenylenediamine mediated by carbon dot-decorated Fe<sub>3</sub>O<sub>4</sub> nanoparticles.

Analytica chimica acta·2026
Same author

A Ruthenium-BODIPY Photosensitizer for Light-Triggered Apoptosis in Triple-Negative Breast Cancer Cells.

Inorganic chemistry·2026
Same author

Facile access to thioethers using sodium thiosulfate as the sulfur surrogate under base-mediated conditions.

Organic & biomolecular chemistry·2026
Same author

Modular Click Assembly of Trivalent Scaffolds Displaying Homogeneous and Mixed Human Milk Oligosaccharide Motifs.

Chemistry, an Asian journal·2026
Same author

High-performance liquid chromatography enables purification of red-emitting carbon dots for cellular imaging.

Analytica chimica acta·2026
Same author

Harnessing Transformation of Metal-Ligand Coordination in Dinuclear Ni(II)-Schiff Base Coordination Polymer for Promoting Electrochemical Oxygen Evolution.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same journal

Selective Degradation of Polyurethanes in Mixed Plastic Wastes via Ir-Catalyzed Hydrogenolysis.

Angewandte Chemie (International ed. in English)·2026
Same journal

Covalent Organic Framework Photocatalysts: Decoding Linkage Chemistry in Hydrogen Peroxide Synthesis From Air and Water.

Angewandte Chemie (International ed. in English)·2026
Same journal

Anomeric Amide Enabled Divergent Synthesis of Unsymmetrical Ureas, Carbamates, Thioesters, and Amides From Aldehydes.

Angewandte Chemie (International ed. in English)·2026
Same journal

Anisotropic Magneto-Chiral Dichroism in Lanthanide Complexes.

Angewandte Chemie (International ed. in English)·2026
Same journal

Engineering LE-CT State Synergy in Aminoboranes for Single Molecule White Light Emission and Dual-Mode Chiroptical/Phosphorescence Output.

Angewandte Chemie (International ed. in English)·2026
Same journal

Editable Hydrogen Bond Network Within the Electric Double Layer for CO<sub>2</sub> Reduction.

Angewandte Chemie (International ed. in English)·2026
See all related articles

Related Experiment Video

Updated: Mar 14, 2026

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

12.1K

Decoding of Carbohydrates With Monosaccharide-Level Resolution Using Tip-Enhanced Raman Scattering.

Jia-Lin Fang1, Ricky Yu-Syun Fan2, Chin-Yu Liang1

  • 1Department of Chemistry and Biochemistry, National Chung Cheng University, Chiayi, 621301, Taiwan.

Angewandte Chemie (International Ed. in English)
|March 13, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a tip-enhanced Raman spectroscopy (TERS) platform for label-free oligosaccharide analysis. The system achieves high sensitivity and specificity in distinguishing glycan structures and monitoring synthesis.

Keywords:
carbohydratesenzymatic glycosylationglycan structural analysisnanopillar trappingtip‐enhanced Raman spectroscopy

More Related Videos

An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry
07:12

An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

Published on: July 29, 2018

7.5K
Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS
12:56

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS

Published on: October 17, 2010

14.1K

Related Experiment Videos

Last Updated: Mar 14, 2026

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

12.1K
An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry
07:12

An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

Published on: July 29, 2018

7.5K
Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS
12:56

Differential Imaging of Biological Structures with Doubly-resonant Coherent Anti-stokes Raman Scattering CARS

Published on: October 17, 2010

14.1K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy

Background:

  • Oligosaccharide analysis is crucial for understanding biological processes.
  • Existing methods often lack sensitivity, specificity, or require labeling.

Purpose of the Study:

  • To develop a high-sensitivity, label-free method for oligosaccharide analysis and synthesis monitoring.
  • To leverage tip-enhanced Raman spectroscopy (TERS) with nanopillar trapping for enhanced glycan detection.

Main Methods:

  • Integration of TERS with nanopillar-assisted glycan trapping.
  • Utilizing mechanical confinement to generate plasmonic hot spots for signal amplification.
  • Employing an azido tag for internal signal normalization.

Main Results:

  • Achieved high sensitivity and reproducibility in vibrational spectra of oligosaccharides.
  • Resolved subtle structural differences, including glycosidic linkage types (β(1→3) vs. β(1→4)).
  • Enabled semi-quantitative estimation of oligosaccharide chain length and real-time monitoring of enzymatic glycosylation, such as fucosylation.

Conclusions:

  • The developed TERS platform offers a powerful tool for label-free glycan sequence analysis.
  • Demonstrated capability for dynamic monitoring of carbohydrate synthesis with high molecular specificity.
  • Establishes TERS as a valuable technique for detailed analysis of complex carbohydrates.