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Matrix-Assisted Laser Desorption Ionization (MALDI)

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Lipidomic Profiling of Rabbit VX2 Tumors Using Matrix-assisted Laser Desorption Ionization Mass Spectrometry Imaging.

Mohammad Mahdi Khavandi¹, A Colleen Crouch², Danielle L Stolley³

¹Department of Interventional Radiology, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Unit 1471, Houston, TX 77030.

Radiology. Imaging Cancer

|March 13, 2026

Summary

Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) revealed distinct lipidomic profiles in VX2 rabbit tumors. These findings highlight microenvironment-driven lipid plasticity in tumor progression.

Keywords:

Ablation Techniques Lipidomic Profiles Mass Spectrometry Imaging VX2 Rabbit Tumor Model

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Area of Science:

Oncology
Biochemistry
Analytical Chemistry

Background:

The VX2 rabbit tumor model is utilized to study tumor progression and therapeutic responses.
Understanding lipidomic profiles within tumors is crucial for deciphering tumor biology and identifying potential biomarkers.
Tumor microenvironments can influence cellular metabolism and lipid composition, leading to site-specific alterations.

Purpose of the Study:

To characterize the lipidomic profiles of VX2 rabbit tumors.
To assess potential lipid plasticity across different tumor microenvironments (liver and flank).
To spatially map lipid distributions within the tumor tissues.

Main Methods:

VX2 tumors were implanted in rabbit livers and flanks.
Tumor tissues were analyzed using matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI).
Lipid identities were confirmed using tandem mass spectrometry and database matching, with spatial correlation to histology.

Main Results:

MALDI-MSI revealed distinct lipidomic profiles between liver and flank VX2 tumors.
Oleate was consistently found in viable tumor regions across both sites.
Site-specific lipids were identified, such as phosphatidic acid in liver tumors and phosphatidylinositol in flank tumors.

Conclusions:

MALDI-MSI successfully mapped lipid distributions within VX2 tumors, showing shared and site-specific alterations.
The study demonstrates heterogeneous spatial lipid distributions in the VX2 model.
Findings suggest microenvironment-driven lipid plasticity in tumors.