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Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Mar 15, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment

Published on: January 6, 2026

Wash-Free Multi-Target Super-Resolution Microscopy With Photocaged DNA Labels.

Nina Kaltenschnee¹, Marina S Dietz², Laurell F Kessler²

¹Institute of Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.

Angewandte Chemie (International Ed. in English)

|March 13, 2026

Summary

This summary is machine-generated.

PhotoPAINT is a novel wash-free method for super-resolution microscopy. This technique uses light-activated DNA labels for accurate multi-target imaging without sample interference.

Keywords:

DNA‐PAINT light control multiplexed imaging photocages single‐molecule localization microscopy

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Last Updated: Mar 15, 2026

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Area of Science:

Cell biology
Microscopy
Molecular imaging

Background:

Super-resolution microscopy enables detailed visualization of cellular structures.
Current DNA-fluorophore labeling methods require direct sample interaction, risking nanometer-scale data accuracy.
There is a need for non-invasive labeling techniques to preserve sample integrity during multi-target imaging.

Purpose of the Study:

To introduce PhotoPAINT, a wash-free method for light-modulated DNA-fluorophore labeling.
To overcome the limitations of direct sample manipulation in super-resolution microscopy.
To enable accurate and efficient multi-target imaging of cellular samples.

Main Methods:

Development of DNA oligonucleotides functionalized with photocaging groups.
Utilizing light irradiation to remove photocaging groups and modulate DNA label hybridization.
Application of PhotoPAINT in confocal, single-molecule localization microscopy (SMLM), and stimulated emission depletion (STED) microscopy.

Main Results:

Demonstrated successful light-modulated targeting of cellular components using PhotoPAINT.
Validated the wash-free nature of the method, preserving sample integrity.
Achieved high-resolution imaging of various cellular targets across different microscopy techniques.

Conclusions:

PhotoPAINT offers a significant advancement in super-resolution microscopy by enabling non-invasive, light-controlled DNA-fluorophore labeling.
The method enhances imaging accuracy and facilitates multi-target analysis without compromising sample nanostructure.
PhotoPAINT is a versatile technique applicable to diverse cellular imaging applications.