Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.8K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.8K
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

2.0K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
2.0K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

7.4K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
7.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Quantitative Host Cell Protein Analysis of Antibody-Based Protein Therapeutics Using the Orbitrap Astral Mass Spectrometer.

Journal of the American Society for Mass Spectrometry·2026
Same author

Native Top-Down Mass Spectrometry Combined with High-Resolution Charge Variant Analysis of Trastuzumab Originator and Biosimilars.

Journal of the American Society for Mass Spectrometry·2026
Same author

Investigation into Clearance of Organic Compounds from Biomanufacturing Process Streams during Ultrafiltration/Diafiltration.

Pharmaceutical research·2026
Same author

Assessing effects of leachables in single-use systems used in cell therapy manufacture.

International journal of pharmaceutics·2025
Same author

Characterisation of small RNA-based therapeutics and their process impurities by fast and sensitive liquid chromatography high resolution mass spectrometry.

Journal of pharmaceutical and biomedical analysis·2025
Same author

Understanding the clearance behaviour of elemental leachables during ultrafiltration/diafiltration from process streams.

Journal of pharmaceutical and biomedical analysis·2025

Related Experiment Video

Updated: Mar 15, 2026

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
11:09

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

10.7K

High-sensitivity MAM to support early-stage activities during biotherapeutic development.

Silvia Millán-Martín1, Anna Mulligan2, Lisa Füssl1

  • 1Characterisation and Comparability Laboratory, National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Blackrock, Co. Dublin, A94 X099, Ireland.

Journal of Chromatography. A
|March 13, 2026
PubMed
Summary

A novel nano-flow liquid chromatography-tandem mass spectrometry (LC-MS/MS) multi-attribute method (MAM) offers sensitive and robust characterization of biotherapeutics. This method enables early-stage bioprocess development and quality control applications.

Keywords:
Critical quality attributesMonoclonal antibodyMulti-attribute methodNISTmAbNew peak detectionPeptide mappingnano-flow LC-MS

More Related Videos

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
07:55

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Published on: June 21, 2019

6.2K
Author Spotlight: Advancing Biotherapeutic Mass Calculation by Introducing mAbScale, a Python-Based Desktop Application
04:24

Author Spotlight: Advancing Biotherapeutic Mass Calculation by Introducing mAbScale, a Python-Based Desktop Application

Published on: June 16, 2023

2.5K

Related Experiment Videos

Last Updated: Mar 15, 2026

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
11:09

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

10.7K
Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
07:55

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Published on: June 21, 2019

6.2K
Author Spotlight: Advancing Biotherapeutic Mass Calculation by Introducing mAbScale, a Python-Based Desktop Application
04:24

Author Spotlight: Advancing Biotherapeutic Mass Calculation by Introducing mAbScale, a Python-Based Desktop Application

Published on: June 16, 2023

2.5K

Area of Science:

  • Biopharmaceutical Analysis
  • Analytical Chemistry
  • Mass Spectrometry

Background:

  • Developing sensitive analytical methods for early-stage biotherapeutic development is challenging due to limited product knowledge and sample availability.
  • Robust methods are crucial for candidate screening, process development, formulation, and stability studies.

Purpose of the Study:

  • To present a high-sensitivity multi-attribute method (MAM) using nano-flow LC-MS/MS for characterizing monoclonal antibody (mAb)-based molecules.
  • To evaluate the method's performance for detecting and quantifying product quality attributes (PQAs) and impurities.

Main Methods:

  • Utilized nano-flow liquid chromatography-tandem mass spectrometry (LC-MS/MS) for high-resolution analysis.
  • Employed Orbitrap-based mass detection within a CFR Part 11 compliant data system.
  • Evaluated run-to-run variation, sensitivity (LOD/LOQ), and new peak detection (NPD) capabilities.

Main Results:

  • Demonstrated low run-to-run variation for key analytical parameters using NISTmAb as a reference.
  • Achieved high sensitivity (LOD/LOQ: 0.6-7.0 ng) for detecting low-abundance PQAs like deamidation and oxidation.
  • Successfully applied the nano-flow MAM for in-process monitoring of PQAs and impurities in a chimeric IgG1 mAb.

Conclusions:

  • The nano-flow MAM is a sensitive and robust approach for biotherapeutic characterization.
  • The method is suitable for early-stage bioprocess development, offering potential for quality control (QC) applications.
  • NPD capability aids in detecting low-level impurities during in-process testing.