Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Science Outreach: Providing an Authentic Independent Research Opportunity in Materials Science to School Students.

Journal of chemical education·2026

Same author

Cross-Platform Assessment of Sub-50 nm Nanopipette Emitters for Native Electrospray Ionization Mass Spectrometry.

bioRxiv : the preprint server for biology·2026

Same author

Nanopipettes Enable Native Mass Spectrometry Studies of the Intrinsically Disordered Protein α-Synuclein in Biochemical Buffers.

Analytical chemistry·2026

Same author

Engineered Coiled-Coils Convert Cholera Toxin B-Pentamers into Programmable Membrane Fusogens.

ACS nano·2026

Same author

Electronic Actuation of Surface-Immobilized, pH-Responsive DNA Nanoswitches.

ACS applied materials & interfaces·2026

Same author

Nanomaterial Modification of Ultramicroelectrodes Using Design-of-Experiments Principles.

ACS electrochemistry·2026

Same journal

Efficient Syngas Photoproduction Enabled by Electronic Engineering of Co-Immobilized Imine COFs.

Angewandte Chemie (International ed. in English)·2026

Same journal

Pathway Controlled Phase Separation of Minimal Building Blocks Utilizing a Dissociative Chemical Transformation.

Angewandte Chemie (International ed. in English)·2026

Same journal

Interaction Hierarchy and Polymorphic Structure-Property Dynamics in Luminescent Molecular Crystals.

Angewandte Chemie (International ed. in English)·2026

Same journal

The Role of Zn-Hf Site Proximity and Oxygen Vacancies for Methanol Formation Over ZnHfO<sub>x</sub> Catalysts Under CO<sub>2</sub> Hydrogenation Conditions.

Angewandte Chemie (International ed. in English)·2026

Same journal

Breaking the Linear Scaling Relationship: Bioinspired Electronic Coupling in S-Bridged Fe-Fe Dual Sites for Efficient Oxygen Reduction.

Angewandte Chemie (International ed. in English)·2026

Same journal

Programming Bio-Bio Electronic Interfaces for Light-Driven Interspecies Electron Transfer.

Angewandte Chemie (International ed. in English)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 18, 2026

Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation

Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation

Published on: July 23, 2015

Visualizing and Quantifying microRNA-Induced DNA Origami Separation at the Nanoscale.

Chalmers C C Chau^1,2, Varun Gupta^2,3, George R Heath^2,3,4

¹School of Electronic and Electrical Engineering, University of Leeds, Leeds, UK.

Angewandte Chemie (International Ed. in English)

|March 13, 2026

Summary

This summary is machine-generated.

This study introduces a novel DNA origami method for detecting circulating microRNAs (miRNAs) even in the presence of RNases. This approach overcomes limitations of current methods, enabling robust small RNA detection in complex biological samples.

Keywords:

DNA origami RNA high speed AFM nanopore single molecule toehold‐mediated strand displacement

More Related Videos

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Published on: October 15, 2019

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

Related Experiment Videos

Last Updated: Jun 18, 2026

Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation

Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation

Published on: July 23, 2015

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Production of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation

Published on: October 15, 2019

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Author Spotlight: Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

Area of Science:

Biotechnology
Molecular Biology
Nanotechnology

Background:

Circulating microRNAs (miRNAs) are valuable disease biomarkers.
Current miRNA detection methods using nanopores are hindered by miRNA instability and RNase activity.
Carrier molecules used in nanopore detection can be degraded by RNases, leading to false negatives.

Purpose of the Study:

To develop a robust method for detecting circulating microRNAs (miRNAs) resistant to RNase degradation.
To utilize DNA origami and toehold-mediated strand displacement (TMSD) for sensitive and specific miRNA detection.
To demonstrate multiplexed miRNA detection in complex biological samples.

Main Methods:

Designed a symmetric DNA origami dimer that disassembles into monomers via TMSD.
Used miRNAs as invading strands to trigger TMSD-driven dimer separation.
Visualized real-time TMSD dynamics using high-speed atomic force microscopy (HS-AFM).
Employed single-molecule nanopore sensing for quantitative endpoint analysis of dimer-to-monomer ratios.

Main Results:

Successfully visualized the nanoscale mechanical dynamics of TMSD using HS-AFM.
Achieved quantitative endpoint analysis of dimer disassembly via single-molecule nanopore sensing.
Demonstrated multiplexed detection of miRNAs.
Successfully detected miRNAs in crude RNA tissue extracts despite RNase presence, showcasing robustness.

Conclusions:

The DNA origami disassembly approach driven by TMSD offers a robust method for small RNA detection in degrading environments.
This technique overcomes the limitations of RNase sensitivity in current miRNA biomarker detection strategies.
The combination of HS-AFM and nanopore sensing provides powerful tools for studying nanoscale molecular dynamics and enabling sensitive biomarker detection.