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Blood Studies for Cardiovascular System I: Cardiac Biomarkers01:20

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Related Experiment Video

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An Integrated Approach for Microprotein Identification and Sequence Analysis
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Integrative Proteomic and Transcriptomic Profiling Identifies Candidate Biomarkers for Discriminating Anaphylactic

Zhi-Hao Fan1, Zi-Qi Yue1, Zi-Kang Liu1

  • 1Department of Forensic Medicine, School of Basic Medical Sciences, Harbin Medical University, Harbin 150081, China.

International Journal of Molecular Sciences
|March 14, 2026
PubMed
Summary

This study identifies key protein biomarkers, fibronectin 1 (FN1), GP1BA, and PF4, to differentiate sudden cardiac death from coronary heart disease and anaphylactic sudden death in forensic investigations.

Keywords:
anaphylactic sudden deathforensic identificationidentification markerproteomicssudden death from coronary heart disease

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Area of Science:

  • Forensic pathology
  • Biomarker discovery
  • Molecular diagnostics

Background:

  • Distinguishing Anaphylactic Sudden Death (ASD) from Sudden Death from Coronary Heart Disease (SD-CHD) presents a forensic challenge.
  • Existing diagnostic methods require improvement for complex cases involving co-existing conditions.

Purpose of the Study:

  • To identify and validate protein biomarkers for differentiating ASD from SD-CHD.
  • To establish a molecular diagnostic strategy for complex sudden death cases.

Main Methods:

  • Established mouse models for Atherosclerosis (AS) and Anaphylaxis (AP).
  • Utilized LC-MS/MS serum proteomic analysis to identify candidate biomarkers.
  • Validated biomarkers using PRM, ELISA, and IHC in mouse models and human tissues.

Main Results:

  • Identified FN1, GP1BA, and PF4 as candidate biomarkers.
  • In mice, serum FN1, GP1BA, and PF4 were elevated in AS, while FN1 was downregulated in AP.
  • Human myocardial tissue showed upregulation of FN1, GP1BA, and PF4 in SD-CHD, with FN1 elevation being most significant.
  • Human airway epithelium showed FN1 upregulation in ASD and ASD+CAS, and GP1BA downregulation.

Conclusions:

  • FN1 is a key differential serum biomarker in mice.
  • PF4 and GP1BA aid in diagnosing SD-CHD in human myocardial tissue.
  • A multi-marker, multi-level approach offers a molecular strategy for forensic identification of complex sudden deaths.