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Fluorescent Labeling Methods for Brain Structure Research.

Chunguang Yin1, Jiangcan Li1, Keyu Meng1

  • 1State Key Laboratory of Chemo and Biosensing, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.

Molecules (Basel, Switzerland)
|March 14, 2026
PubMed
Summary

This review explores advanced fluorescent labeling and imaging techniques for analyzing brain structure and function. These innovations offer new tools for understanding neurological disorders and guiding brain tumor treatments.

Keywords:
brain imagingfluorescent labelingimmunofluorescenceneural circuitsviral tracing

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Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Medical Imaging

Background:

  • The brain's complex structural network necessitates advanced analytical tools.
  • Fluorescent labeling and imaging are crucial for multiscale brain anatomy analysis.
  • Understanding brain function and neurological disorders requires detailed anatomical insights.

Purpose of the Study:

  • To review technological advances in fluorescent labeling for neuroscience.
  • To highlight applications in neural circuit analysis, cerebrovascular imaging, and neuronal activity monitoring.
  • To discuss fluorescence-guided treatment of brain tumors.

Main Methods:

  • Summarization of technological advancements in fluorescent labeling.
  • Review of applications in various neuroscience research areas.
  • Discussion of integrated approaches like tissue clearing and AI-assisted processing.

Main Results:

  • Technological progress enables comprehensive analysis of brain anatomy.
  • Applications span neural circuits, blood vessels, neuronal activity, and tumor treatment.
  • Integration of smart labeling with advanced imaging and AI is a key trend.

Conclusions:

  • Advanced fluorescent techniques provide critical tools for neuroscience.
  • Future directions involve combining high-resolution imaging with big data analysis.
  • These tools are vital for understanding brain structure, function, and disease.