Canine Adipose MSC-Derived Exosomes Ameliorate Skeletal Muscle Injury in Mice

Area of Science:

• Regenerative Medicine
• Cell Biology
• Veterinary Medicine

Background:

• Severe skeletal muscle injuries in dogs lead to atrophy, fibrosis, and fat accumulation, causing dysfunction and reduced quality of life.
• Current treatments for such conditions are ineffective.
• Canine adipose mesenchymal stem cell-derived exosomes (cADMSC-Exos) are being explored as a novel acellular therapy.

Purpose of the Study:

• To investigate the therapeutic potential of cADMSC-Exos for repairing muscle atrophy and injury.
• To evaluate the effects of cADMSC-Exos on muscle atrophy in vitro and in vivo.
• To assess the efficacy and safety of cADMSC-Exos in preclinical models of muscle damage.

Main Methods:

• Isolation and characterization of cADMSCs and cADMSC-Exos.
• Establishment of a dexamethasone-induced C2C12 myotube atrophy model to assess anti-atrophic effects.
• Construction of a glycerol-induced mouse muscle injury model for in vivo evaluation.
• Histological analysis and Western blot to evaluate efficacy and safety.

Main Results:

• cADMSC-Exos exhibited significant anti-atrophic activity in both in vitro and in vivo models.
• Exosomes ameliorated skeletal muscle atrophy and reduced the expression of key atrophy-related factors, MuRF1 and Atrogin-1.
• cADMSC-Exos markedly alleviated fibrosis and fatty infiltration in injured muscle tissue.

Conclusions:

• cADMSC-Exos promote muscle repair and attenuate pathological remodeling in muscle disorders.
• The exosomes modulate the local microenvironment and protein expression to achieve therapeutic effects.
• These findings highlight cADMSC-Exos as a promising acellular therapeutic strategy for muscular disorders.