Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pinewood Biochar as an Affordable Adsorbent for Short- and Medium-Chain PFAS Removal from Contaminated Water.

Molecules (Basel, Switzerland)·2026
Same author

Recent Advances in the Detection of Food Contaminants and Pollutants.

Foods (Basel, Switzerland)·2026
Same author

Plastic Additives in NIST Standard Reference Material 2585 and Settled Dust From Indoor Domestic and Working Environments.

Journal of AOAC International·2025
Same author

Phthalate and non-phthalate plastic additives associated with size segregated airborne particles collected in e-waste recycling plants.

Environmental science and pollution research international·2025
Same author

Comparison of Capillary Electrophoresis and HPLC-Based Methods in the Monitoring of Moniliformin in Maize.

Foods (Basel, Switzerland)·2025
Same author

Exploring the Composition of Blueberry-Based Functional Products: Polyphenolic and Elemental Characterization and Quantification.

Foods (Basel, Switzerland)·2025

Related Experiment Video

Updated: Jun 25, 2026

Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
14:39

Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults

Published on: April 22, 2022

4.5K

A Sustainable CE-DAD Screening Method for Multi-Class Polyphenol Profiling in Rosehip-Based Herbal Tea and

Giulia Simonetti1, Francesca Buiarelli1, Sara Astolfi1

  • 1Department of Chemistry, "Sapienza" University of Rome, Piazzale Aldo Moro, 5, 00185 Rome, Italy.

Foods (Basel, Switzerland)
|March 14, 2026
PubMed
Summary

A new green analytical method using capillary zone electrophoresis and diode array detection (CE-DAD) efficiently screens polyphenols in rosehip products. This sustainable approach offers accurate quality control and functional insights into health-promoting compounds.

Keywords:
Blue Applicability Grade Indexantioxidant propertiescapillary zone electrophoresisgreen chemistrypolyphenols profilingrosehip herbal teasupplement

More Related Videos

Source and Route of Pyrrolizidine Alkaloid Contamination in Tea Samples
06:04

Source and Route of Pyrrolizidine Alkaloid Contamination in Tea Samples

Published on: September 28, 2022

2.6K
Author Spotlight: Eco-Friendly Extraction of Bioactive Compounds Using Polyol-Based Microwave-Assisted Techniques
07:05

Author Spotlight: Eco-Friendly Extraction of Bioactive Compounds Using Polyol-Based Microwave-Assisted Techniques

Published on: August 23, 2024

3.1K

Related Experiment Videos

Last Updated: Jun 25, 2026

Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults
14:39

Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults

Published on: April 22, 2022

4.5K
Source and Route of Pyrrolizidine Alkaloid Contamination in Tea Samples
06:04

Source and Route of Pyrrolizidine Alkaloid Contamination in Tea Samples

Published on: September 28, 2022

2.6K
Author Spotlight: Eco-Friendly Extraction of Bioactive Compounds Using Polyol-Based Microwave-Assisted Techniques
07:05

Author Spotlight: Eco-Friendly Extraction of Bioactive Compounds Using Polyol-Based Microwave-Assisted Techniques

Published on: August 23, 2024

3.1K

Area of Science:

  • Analytical Chemistry
  • Green Chemistry
  • Food Science

Background:

  • Rosehip products contain beneficial polyphenols, necessitating robust quality control methods.
  • Conventional polyphenol analysis is often resource-intensive and environmentally taxing.
  • Developing sustainable analytical techniques is crucial for evaluating rosehip product quality and function.

Purpose of the Study:

  • To develop and validate a green, accessible analytical method for polyphenol profiling in rosehip products.
  • To establish capillary zone electrophoresis coupled with diode array detection (CE-DAD) as a sustainable screening tool.
  • To correlate polyphenol composition with antioxidant activity for functional interpretation.

Main Methods:

  • Optimization of capillary zone electrophoresis (CE) conditions (buffer pH, voltage, electrolyte concentration) using twelve standard polyphenols.
  • Analysis of herbal tea and supplement samples using CE-DAD, with and without a cartridge-based clean-up step.
  • Quantification of identified polyphenols and assessment of antioxidant capacity via DPPH and ABTS assays.

Main Results:

  • The developed CE-DAD method demonstrated excellent linearity (R² > 0.99), reproducibility (RSD 0.8-1.6%), and sensitivity (LOD 0.4-1.4 μg/mL).
  • Eight target polyphenols were successfully identified and quantified in real rosehip product samples.
  • Herbal tea exhibited higher antioxidant activity (13.8 mg Trolox/g DPPH, 15.3 mg Trolox/g ABTS) compared to the food supplement (7.4 mg Trolox/g DPPH, 7.4 mg Trolox/g ABTS).

Conclusions:

  • The CE-DAD method provides a sustainable, efficient, and accurate approach for polyphenol analysis in rosehip-based products.
  • The developed method facilitates simultaneous profiling of diverse polyphenol classes, aiding quality control.
  • The study confirms the method's low environmental impact and applicability for functional assessment of rosehip products.