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Integrated Metabolome-Transcriptome Profiling Identifies JrMYB8 as a Repressor of Polyphenol Biosynthesis in Walnut
Fang Sheng1,2, Qiang Jin1, Cuiyun Wu1
1College of Horticulture and Forestry, Tarim University, Alar 843300, China.
Plants (Basel, Switzerland)
|March 14, 2026
Summary
Walnut husk and pellicle metabolites were analyzed using multi-omics. A key regulator, JrMYB8, was identified, offering a target for improving walnut flavor and polyphenol content.
Area of Science:
- Plant biochemistry
- Molecular biology
- Nutritional science
Background:
- Walnuts are rich in bioactive polyphenols.
- Comprehensive metabolite analysis of walnut husk and pellicle is lacking.
Purpose of the Study:
- To characterize metabolite profiles and regulatory networks in walnut husk and pellicle.
- To identify molecular targets for polyphenol manipulation in walnuts.
Main Methods:
- Multi-omics approaches: untargeted metabolomics and transcriptome analysis.
- Co-expression network analysis to identify regulatory factors.
- Functional characterization of identified genes.
Main Results:
- Distinct polyphenol profiles in husk (phenolic acids) and pellicle (flavonoids).
- Identification of transcription factor JrMYB8 as a polyphenol biosynthesis regulator.
- JrMYB8 overexpression repressed phenolic acid and flavonoid accumulation by targeting JrC4H.
Conclusions:
- JrMYB8 is a key regulator of polyphenol biosynthesis in walnuts.
- Findings provide molecular targets for enhancing walnut polyphenol content and flavor.
- Potential for improving walnut breeding programs through targeted gene manipulation.
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