Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Testing a Claim about Mean: Known Population SD01:11

Testing a Claim about Mean: Known Population SD

3.3K
A complete procedure of testing the hypothesis about a population mean is explained here.
Estimating a population mean requires the samples to be distributed normally. The data should be collected from the randomly selected samples having no sampling bias. The sample size needed to be higher than 30, and most importantly, the population standard deviation should be already known.
In most realistic situations, the population standard deviation is often unknown, but in rare circumstances, when it...
3.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Bone and Radiologic Findings in Congenital Generalized Lipodystrophy: A Systematic Review and Report of 60 Cases.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research·2026
Same author

Type B insulin resistance with glycemic extremes: a case report and literature review.

Frontiers in endocrinology·2026
Same author

Case Report: Twenty years of metreleptin therapy in congenital generalized lipodystrophy type 1: the longest reported follow-up to date.

Frontiers in endocrinology·2026
Same author

A review of Leydig cell hyperplasia in fish models: Implications for endocrine toxicity studies.

Regulatory toxicology and pharmacology : RTP·2026
Same author

Absolute Versus Relative Approaches to Severity Scoring of Histopathologic Findings in Pre-Clinical Toxicology Studies: What's the Difference, and How, When, and Why This Matters.

Toxicologic pathology·2026
Same author

A framework to Identify High-Priority Legacy Active Pharmaceutical Ingredients for Environmental Risk Assessment.

Integrated environmental assessment and management·2026

Related Experiment Video

Updated: Mar 15, 2026

Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri
09:43

Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri

Published on: April 24, 2018

8.2K

The fathead minnow extended one generation reproduction test (FEOGRT): A design for regulatory application.

Grace H Panter1, Rebecca J Brown1, Jeffrey C Wolf2

  • 1wca environment Ltd, Faringdon, United Kingdom.

Integrated Environmental Assessment and Management
|March 14, 2026
PubMed
Summary

The fathead minnow extended one generation reproduction test (FEOGRT) is a robust, regulatory-acceptable method for assessing fish reproductive and endocrine toxicity. This test offers advantages over other extended one-generation reproduction tests, leveraging existing data and species suitability.

Keywords:
endocrineenvironmental risk assessmentextended one generation reproduction testfathead minnowlife-cycle

More Related Videos

Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine
08:33

Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine

Published on: July 24, 2018

11.9K
A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis
06:59

A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis

Published on: August 11, 2010

12.5K

Related Experiment Videos

Last Updated: Mar 15, 2026

Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri
09:43

Protocol for Acute and Chronic Ecotoxicity Testing of the Turquoise Killifish Nothobranchius furzeri

Published on: April 24, 2018

8.2K
Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine
08:33

Experimental Protocol for Examining Behavioral Response Profiles in Larval Fish: Application to the Neuro-stimulant Caffeine

Published on: July 24, 2018

11.9K
A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis
06:59

A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis

Published on: August 11, 2010

12.5K

Area of Science:

  • Aquatic toxicology
  • Ecotoxicology
  • Endocrinology

Background:

  • Fathead minnow (Pimephales promelas) is a standard test species for aquatic toxicology.
  • Extended one-generation reproduction tests (EOGRTs) assess toxicity across multiple generations.
  • Existing data and ease of use make fathead minnow suitable for regulatory testing.

Purpose of the Study:

  • To evaluate the fathead minnow extended one generation reproduction test (FEOGRT) as a regulatory-acceptable method.
  • To compare FEOGRT with similar tests using Japanese medaka (MEOGRT) and zebrafish (ZEOGRT).
  • To highlight the advantages of FEOGRT for assessing reproductive and endocrine toxicity.

Main Methods:

  • The FEOGRT incorporates key life stages and endpoints across three generations.
  • Mechanistic data includes vitellogenin, sex characteristics, sex ratio, and gonadal histopathology.
  • Adversity parameters include growth, development, and reproduction.

Main Results:

  • FEOGRT is comparable to MEOGRT and ZEOGRT in detecting transgenerational toxicity.
  • FEOGRT offers advantages including species concordance and utilization of existing data, reducing the need for range-finding studies.
  • Fathead minnow's ease of culture, handling, and established validation support FEOGRT's utility.

Conclusions:

  • The FEOGRT is a robust and regulatory-acceptable test for assessing fish reproductive and endocrine toxicity.
  • FEOGRT provides a valuable approach for detecting both manifest and potential transgenerational toxicity.
  • The test design leverages the fathead minnow's suitability as a model organism in aquatic toxicology.