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Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Induced Pluripotent Stem Cells01:06

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Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
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Induced Pluripotent Stem Cells01:13

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Related Experiment Video

Updated: Mar 15, 2026

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
10:24

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells

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Poking Pluripotency: Nanoinjection Into Human iPSCs.

Jann Harberts1,2, Vuong Thi Thanh Xuan Ho1,2, Yuqi Yang2,3

  • 1Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, VIC, Australia.

Advanced Materials (Deerfield Beach, Fla.)
|March 14, 2026
PubMed
Summary

Nanotube nanoinjection offers a safe, efficient non-viral method for delivering messenger RNA (mRNA) into human induced pluripotent stem cells (hiPSCs). This technique maintains hiPSC pluripotency and differentiation capacity, advancing regenerative medicine and disease modeling applications.

Keywords:
human induced pluripotent stem cellsmRNAnanoinjectionnanoneedlessilicon nanotubestransfection

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Area of Science:

  • Biotechnology and Regenerative Medicine
  • Cellular Engineering
  • Nanotechnology

Background:

  • Human induced pluripotent stem cells (hiPSCs) are crucial for regenerative medicine and disease modeling.
  • Current genetic material delivery methods (viral, electroporation) have safety, precision, and cytotoxicity limitations.
  • Efficient and safe delivery of exogenous genetic material into hiPSCs is essential for their application.

Purpose of the Study:

  • To introduce nanotube (NT)-mediated nanoinjection as a novel, non-viral strategy for functional messenger RNA (mRNA) delivery into hiPSCs.
  • To demonstrate the efficacy and safety of NT-mediated nanoinjection in hiPSCs, preserving their pluripotent state.

Main Methods:

  • Developed re-engineered NTs with enhanced cargo capacity and cell interfacing geometry.
  • Implemented delayed extracellular matrix (ECM) application and advanced surface engineering for hiPSC nanoinjection.
  • Delivered various mRNA payloads (mCherry, GFP, YPet) into hiPSCs via nanoinjection.

Main Results:

  • Achieved effective nanoinjection into hiPSCs with average transfection yields of ~55% to ~64% for single mRNAs.
  • Demonstrated successful co-transfection of mCherry and GFP mRNA with yields of ~61%.
  • Confirmed hiPSC integrity and pluripotency post-nanoinjection through marker expression (NANOG, OCT4, SOX2) and neuronal differentiation capacity over several passages.

Conclusions:

  • NT-mediated nanoinjection is a powerful and effective non-viral tool for delivering mRNA into hiPSCs.
  • This method maintains hiPSC pluripotency and functional differentiation capabilities.
  • Nanoinjection serves as an enabling platform for precise cellular engineering and advanced stem cell applications.