Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Online image reconstruction via Multiple Orthogonal Reference Sensitivity Encoding (MORSE).

Magma (New York, N.Y.)·2026
Same author

A combination of ketones and NAD<sup>+</sup> precursor preserves white matter integrity in mild cognitive impairment.

Alzheimer's & dementia (New York, N. Y.)·2026
Same author

Language laterality and cognitive skills: does anatomy matter?

Cerebral cortex (New York, N.Y. : 1991)·2026
Same author

Automated Segmentation of Brainstem and Subcortical White Matter: Mapping the Deep Tegmental Core with BundleParc.

bioRxiv : the preprint server for biology·2026
Same author

Disentangling crossing fibers with advanced dMRI methods reveals bundle-specific degeneration across the visual system in asymmetric glaucoma.

PloS one·2026
Same author

Simultaneous Water-Specific and Fat-Specific T1 and Fat Fraction Mapping of the Abdomen in Free Breathing Conditions With a Radially Encoded MP2RAGE.

NMR in biomedicine·2026

Related Experiment Video

Updated: Mar 15, 2026

Simultaneous PET/MRI Imaging During Mouse Cerebral Hypoxia-ischemia
10:35

Simultaneous PET/MRI Imaging During Mouse Cerebral Hypoxia-ischemia

Published on: September 20, 2015

12.8K

An Optimised Multishot EPI Protocol for Ex Vivo Mouse Brain Diffusion MRI With Gd-DOTA Contrast Enhancement.

Elise Cosenza1, Nadège Corbin2,3, Emeline J Ribot2

  • 1Groupe d'Imagerie Neurofonctionnelle, Institut Des Maladies Neurodégénératives (GIN-IMN), UMR5293, CNRS, CEA, Université de Bordeaux, Bordeaux, France.

NMR in Biomedicine
|March 14, 2026
PubMed
Summary

We optimized diffusion MRI in mouse brains using a 3D spin-echo multishot EPI protocol and Gd-DOTA contrast agent. This significantly reduces scan time and improves signal-to-noise ratio for high-quality ex vivo imaging.

Keywords:
Gd‐DOTAdiffusion magnetic resonance imagingex vivo techniquesmouse braintractography

More Related Videos

Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
09:08

Multiple-mouse Neuroanatomical Magnetic Resonance Imaging

Published on: February 27, 2011

16.6K
Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring
17:16

Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring

Published on: December 9, 2010

11.0K

Related Experiment Videos

Last Updated: Mar 15, 2026

Simultaneous PET/MRI Imaging During Mouse Cerebral Hypoxia-ischemia
10:35

Simultaneous PET/MRI Imaging During Mouse Cerebral Hypoxia-ischemia

Published on: September 20, 2015

12.8K
Multiple-mouse Neuroanatomical Magnetic Resonance Imaging
09:08

Multiple-mouse Neuroanatomical Magnetic Resonance Imaging

Published on: February 27, 2011

16.6K
Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring
17:16

Registered Bioimaging of Nanomaterials for Diagnostic and Therapeutic Monitoring

Published on: December 9, 2010

11.0K

Area of Science:

  • Neuroimaging
  • Biomedical Engineering
  • Magnetic Resonance Imaging

Background:

  • Diffusion-weighted MRI (dMRI) in small animal models faces challenges including long acquisition times, low signal-to-noise ratio (SNR), and susceptibility artifacts.
  • Existing methods often require compromises between image quality, acquisition speed, and resolution.

Purpose of the Study:

  • To develop and optimize an efficient, high-quality ex vivo dMRI protocol for mouse brains.
  • To combine a 3D spin-echo multishot EPI (SE-msEPI) sequence with Gd-DOTA contrast agent administration.

Main Methods:

  • Systematic testing of EPI shots to balance artifact reduction, SNR, and scan duration.
  • Optimization of Gd-DOTA incubation concentration and duration to enable shorter repetition times (TR) without compromising diffusion metrics.
  • Acquisition of whole-brain data at 100-μm isotropic resolution with 60 directions (b=3000 s/mm²).

Main Results:

  • An optimal trade-off was achieved with 12 EPI shots.
  • Gd-DOTA incubation (3 mM, 7 days) allowed TR reduction from 1500 ms to 400 ms, increasing SNR threefold.
  • The optimized protocol achieved whole-brain imaging at 100-μm resolution in ~11 hours, a fourfold acceleration.
  • Gd-DOTA enhanced acquisitions showed fractional anisotropy (FA) values consistent with long-TR references, unlike low-SNR acquisitions.
  • Improved image quality and anatomically plausible white matter tract reconstruction were confirmed.

Conclusions:

  • The optimized 3D SE-msEPI protocol combined with Gd-DOTA significantly enhances efficiency and quality of ex vivo mouse brain dMRI.
  • This approach enables high-throughput connectomics and microstructural studies with reduced scan times and improved data reliability.
  • The protocol offers a reproducible framework for increased tissue sample reuse and reduced animal usage in preclinical research.