Evaluation of a field method for qPCR analysis of toxigenic cyanobacteria

Quantitative Polymerase Chain Reaction (qPCR) is a well-established method for molecular detection and quantification of microorganisms. Molecular detection of toxigenic cyanobacteria using qPCR has also been employed as a useful tool for predicting cyanotoxin production and formation of harmful cyanobacterial blooms (cHABs). Recently, portable thermocyclers and extraction procedures and reagents have been developed for onsite application of qPCR analysis. However, use of portable qPCR analysis for the detection and quantification of toxigenic cyanobacteria has not been evaluated. Here, we provide an evaluation of a field method for DNA extraction and qPCR analysis using the Biomeme Franklin thermocycler for detection of toxigenic cyanobacteria. The results obtained from the portable device provide identification and quantification of toxigenic Microcystis and are comparable with lab-based extraction and analysis. This field method for extraction and qPCR analysis will provide alternative research tools to evaluate source and recreational water for toxic cyanobacteria. This field method allows users to obtain immediate on-site results for identification and quantification of toxigenic Microcystis.