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Optimal Se content on P/G-EGCG@Se nanofibers reverses muscle-derived IL-6- induced osteogenesis depression
Weichao Gui1, Yan Deng2, Xiaolong Zhang3
1Division of Orthopaedics and Traumatology, Department of Orthopaedics, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China; Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Department of Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou, 510515, China.
Objective:
To develop electrospun polycaprolactone/gelatin (P/G) nanofibers modified with epigallocatechin gallate‑selenium nanoparticles (EGCG@Se) and evaluate their osteogenic effects in vitro and in vivo.
Methods:
P/G nanofibers were fabricated by electrospinning, and EGCG@Se nanoparticles were synthesized using cysteine as a reducing agent. Composite fibers were formed by surface deposition and characterized using SEM, WCA, EDS, FTIR, AFM, and mechanical tests. In vitro, MSCs and MC3T3-E1 cells were cultured on P/G-EGCG@Se fibers under osteogenic induction. A tibial defect model in KM mice was used for in vivo osteogenic evaluation. Gene and protein expression were analyzed by qPCR, Western blot and ELISA.
Results:
P/G fibers with an 8:2 weight ratio and 12 kV voltage exhibited optimal properties. EGCG@Se enhanced MSCs viability, adhesion and osteogenic differentiation. In vitro, 7.5 μg/mL Se-loaded fibers showed the best osteoinductive effect. However in vivo, 15 μg/mL Se-loaded fibers resulted in the optimal tibial defect repair, which significantly suppressed IL-6 expression in skeletal muscle. In vitro co-culturing test demonstrated that Se content in P/G-EGCG@Se nanofibers controls IL-6 production in muscle fibers.
Conclusions:
The special physicochemical properties of P/G-EGCG@Se fibers, assisted by bone repair effects of EGCG, and by the optimal Se content induced IL-6 level reduction from muscle tissue, contribute to the efficient bone defect healing in vivo.
