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Published on: August 29, 2018
Comparative proteomics analysis of physicochemical variations in squid (Uroteuthis edulis) across simulated
Lukai Ma1, Taiyu Li2, Xianhong Bi1
1College of Light Industry and Food, Zhongkai University of Agriculture and Engineering/Key Laboratory of Green Processing and Intelligent Manufacturing of Lingnan Specialty Food, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology, Guangzhou, China.
Abstract:
This study investigated three key stages of cold-chain logistics-chilling, supercooling, and freezing-to evaluate their effects on the physicochemical properties of squid (Uroteuthis edulis). During chilling, sulfhydryl groups showed increased oxidation and Ca2+-ATPase activity declined markedly in the later storage-stage. Proteomic analysis indicated that prolonged cold-chain duration led to original proteins cleavage, resulting in increased number of differentially expressed proteins (DEPs). Chilled group exhibited a greater number of altered Gene Ontology terms and DEPs than supercooled and frozen groups. Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that higher storage temperatures activate apoptosis- and proteolysis-related pathways, thereby accelerating quality deterioration. Correlation analysis identified 61, 27, and 25 DEPs that were significantly associated with key quality indicators during chilling, supercooling, and freezing, respectively. This study clarifies the molecular mechanisms underlying squid quality deterioration and offers a theoretical basis for developing targeted preservation strategies and optimizing cold-chain process parameters.
