Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

IR Frequency Region: Fingerprint Region01:03

IR Frequency Region: Fingerprint Region

2.2K
IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the...
2.2K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

13.5K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
13.5K
Next-generation Sequencing03:00

Next-generation Sequencing

100.6K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
100.6K
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

833
Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...
833
¹H NMR: Complex Splitting01:13

¹H NMR: Complex Splitting

2.1K
A proton M that is coupled to a proton X results in doublet signals for M. However, NMR-active nuclei can be simultaneously coupled to more than one nonequivalent nucleus. When M is coupled to a second proton A, such as in styrene oxide, each peak in the doublet is split into another doublet.
Splitting diagrams or splitting tree diagrams are routinely used to depict such complex couplings. While drawing splitting diagrams, the splitting with the larger coupling constant is usually applied...
2.1K
Sanger Sequencing01:57

Sanger Sequencing

777.3K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
777.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Physiology-guided Self-supervised Learning for Simultaneous Dual-Tracer PET Separation.

IEEE transactions on medical imaging·2026
Same author

ContiMorph: An unsupervised learning framework for cardiac motion tracking with time-continuous diffeomorphism.

Medical image analysis·2026
Same author

On the Identifiability of Hybrid Deep Generative Models: Meta-Learning as a Solution.

Advances in neural information processing systems·2026
Same author

Mitochondrial quality control in health and disease: Updates 2026.

Chinese medical journal·2026
Same author

PD-1 H (VISTA) drives immunosuppressive reprogramming of glioma-associated myeloid cells to promote glioma progression.

Journal of translational medicine·2026
Same author

Seeing is believing: visualizing spatiotemporal lipophagic flux in vivo with the tfLiveDrop reporter mice.

Autophagy reports·2026
Same journal

A Comparison of Tissue Property Values Estimated Using Conventional Cardiac MRF and MT-Cardiac MRF.

Magnetic resonance in medicine·2026
Same journal

Dependence of the Extra-Cellular Diffusion Coefficient on the Fractions of Neurites and Cell Bodies in Gray Matter.

Magnetic resonance in medicine·2026
Same journal

Triple-Pulse <sup>23</sup>Na MRI Sequence (TriNa) for Simultaneous Acquisition of Spin-Density-Weighted and Fluid-Attenuated Images.

Magnetic resonance in medicine·2026
Same journal

Evaluation of Phantom Doping Materials in Quantitative Susceptibility Mapping.

Magnetic resonance in medicine·2026
Same journal

Design of an 8-Channel Transmit 32-Channel Receive 11.7T Head Coil and Evaluation of SNR Gains.

Magnetic resonance in medicine·2026
Same journal

The Potential for Absolute Temperature Imaging Based on Brain Metabolites Using an FID-Shifting Approach in Gradient Echo Planar Spectroscopic Imaging (GREPSI).

Magnetic resonance in medicine·2026
See all related articles

Related Experiment Video

Updated: Mar 17, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

23.4K

oDual-MRF: An Optimized Dual-Alternating MR Fingerprinting Sequence.

Shizhuo Li1, Bo Zhao2,3, Pengcheng Xu1

  • 1College of Optical Science and Engineering, Zhejiang University, Hangzhou, China.

Magnetic Resonance in Medicine
|March 15, 2026
PubMed
Summary
This summary is machine-generated.

The novel Dual-MRF sequence improves T2 mapping accuracy in magnetic resonance fingerprinting. Optimized Dual-MRF (oDual-MRF) further enhances T2 quantification while maintaining T1 accuracy, offering reliable multiparametric MRF.

Keywords:
Cramer‐Rao BoundMagnetic Resonance Fingerprintingquantitative mapsequence parameter optimization

More Related Videos

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat VNTR - Fragment Length Analysis FLA
09:39

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat VNTR - Fragment Length Analysis FLA

Published on: July 15, 2011

27.9K
Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

1.2K

Related Experiment Videos

Last Updated: Mar 17, 2026

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
08:35

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Published on: July 17, 2021

23.4K
DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat VNTR - Fragment Length Analysis FLA
09:39

DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat VNTR - Fragment Length Analysis FLA

Published on: July 15, 2011

27.9K
Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
07:10

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

1.2K

Area of Science:

  • Magnetic Resonance Imaging (MRI)
  • Quantitative Imaging
  • Biomedical Engineering

Background:

  • Magnetic Resonance Fingerprinting (MRF) enables simultaneous T1 and T2 mapping.
  • Conventional MRF sequences like FISP-MRF face challenges in quantitative accuracy, particularly for T2 values.
  • Improving the precision of MRF parameter quantification is crucial for clinical applications.

Purpose of the Study:

  • To introduce and evaluate the Dual-MRF sequence for enhanced T2 mapping accuracy in MRF.
  • To optimize Dual-MRF parameters using Cramer-Rao Bound (CRB) for minimized estimator variance.
  • To compare the performance of Dual-MRF against conventional FISP-MRF.

Main Methods:

  • Developed the Dual-MRF sequence by alternating FISP and PSIF acquisitions within the MRF framework.
  • Optimized sequence parameters (flip angle, repetition time, alternation scheme) for the oDual-MRF using CRB.
  • Validated sequence performance through extensive simulations, phantom studies, and in vivo experiments.

Main Results:

  • Dual-MRF demonstrated superior T2 quantification accuracy compared to standard FISP-MRF.
  • The optimized oDual-MRF sequence further improved T2 accuracy.
  • Both Dual-MRF and oDual-MRF maintained high T1 accuracy without compromising T1 map quality.

Conclusions:

  • CRB-based optimization of the Dual-MRF sequence significantly enhances T2 quantification accuracy.
  • The proposed method preserves T1 accuracy, enabling reliable multiparametric MRF.
  • Dual-MRF presents a promising advancement for precise quantitative MRI.