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Updated: Mar 17, 2026
A Hydroponic Co-cultivation System for Simultaneous and Systematic Analysis of Plant/Microbe Molecular Interactions and Signaling
Published on: July 22, 2017
Comparative transcriptome and physiological analyses of the Polygonatum odoratum (Mill.) Druce rhizome responses to
1Academician Workstation, Hunan Key Laboratory of the Research and Development of Novel Pharmaceutical, Preparations, Changsha Medical University, Changsha 410219, China; Institute of Chinese Medicine Resources, Hunan Academy of Chinese Medicine, Changsha 410013, China; Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China.
Abstract:
Polygonatum odoratum, a valuable medicinal herb, faces severe cultivation constraints due to continuous cropping (CC) obstacles, yet the physiological and molecular mechanisms in its rhizome remain unclear. This study integrates physiological and transcriptomic analyses comparing CC and first-cropping plants. Under CC stress, rhizomes exhibited reduced diameter, root length, and root number, along with decreased antioxidant enzyme activities and polysaccharide content, but increased malondialdehyde levels and 3 types of phenolic acids. RNA-seq identified 3357 upregulated and 1215 downregulated DEGs. KEGG analysis revealed downregulation in plant hormone signal transduction, particularly in IAA-related genes, while the JA, CTK, and ABA pathways, along with key carbohydrate metabolism processes, were upregulated. Moreover, exogenous NAA application promotes the root growth in the rhizome of P. odoratum. These results uncover hormone signaling reprogramming and metabolic shifts underlying CC responses, offering novel insights and a theoretical basis for mitigating cropping obstacles in medicinal plants.
