Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

12.1K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
12.1K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

17.6K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
17.6K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-fidelity fast fluorescence lifetime imaging by event-based denoising.

Nature biotechnology·2026
Same author

High numerical aperture confocal volumetric mesoscope reveals mesoscale subcellular dynamics in vivo.

Nature biotechnology·2026
Same author

Early Versus Delayed Laparoscopic Repair of Surgically Induced Vesicovaginal Fistula.

Urogynecology (Philadelphia, Pa.)·2026
Same author

Small language models in medicine.

Nature biomedical engineering·2026
Same author

Sex-specific signatures of gut microbiota and systemic inflammation in patients with urolithiasis: a cross-sectional study.

Frontiers in cellular and infection microbiology·2026
Same author

Frequent exposure to biologics is associated with small intestinal bacterial overgrowth in patients with Crohn's disease: a retrospective case-control study.

PeerJ·2026

Related Experiment Video

Updated: Mar 17, 2026

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.7K

Intravital mesoscale optical imaging: challenges, techniques, and future perspectives.

Mingrui Wang1,2, Jiamin Wu3,2, Qionghai Dai3,2

  • 1Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518071, Guangdong, China.

Biophysics Reports
|March 16, 2026
PubMed
Summary

Intravital mesoscale imaging offers high-resolution views across large areas, crucial for biological research. Overcoming challenges like scattering and photobleaching is key to advancing this powerful imaging technique.

Keywords:
Cellular dynamicsIntravital microscopeMesoscale optical imagingOptical aberrationSpace bandwidth productTomography

More Related Videos

Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila
11:51

Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila

Published on: August 20, 2009

11.2K
A Scanning Electron Microscopy-Compatible Optical Imaging Method for Mesoscopic All-Cell Brain Mapping
09:40

A Scanning Electron Microscopy-Compatible Optical Imaging Method for Mesoscopic All-Cell Brain Mapping

Published on: February 20, 2026

189

Related Experiment Videos

Last Updated: Mar 17, 2026

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.7K
Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila
11:51

Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila

Published on: August 20, 2009

11.2K
A Scanning Electron Microscopy-Compatible Optical Imaging Method for Mesoscopic All-Cell Brain Mapping
09:40

A Scanning Electron Microscopy-Compatible Optical Imaging Method for Mesoscopic All-Cell Brain Mapping

Published on: February 20, 2026

189

Area of Science:

  • Biomedical Optics
  • Microscopy
  • Biological Imaging

Background:

  • Intravital mesoscale imaging bridges cellular and organ-level studies with high-resolution, large-field-of-view visualization.
  • Advancements in optical microscopy improve performance, but challenges persist in balancing resolution, speed, and field of view.

Purpose of the Study:

  • To review key challenges in high-performance intravital mesoscale optical imaging.
  • To provide an overview of advanced optical imaging techniques relevant to mesoscale visualization.

Main Methods:

  • Review of wide-field, laser-scanning, and computational imaging approaches.
  • Analysis of limitations including scattering, aberrations, phototoxicity, and photobleaching.

Main Results:

  • Identified critical challenges in achieving optimal spatial resolution, imaging speed, and field of view.
  • Summarized current advanced optical and computational imaging techniques.

Conclusions:

  • Further improvements are needed in imaging depth and space-bandwidth products.
  • Integration of computational methods for real-time processing and data analysis is essential for future advancements.