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Updated: Jul 8, 2026
Analysis of Targeted Viral Protein Nanoparticles Delivered to HER2+ Tumors
Published on: June 18, 2013
DNA Aptamer-Functionalized Fluorescent Silica Nanoparticles: A Robust Strategy for Specific Detection and Bioimaging
Juntao Tan1, Zixi Hu2, Da Huang1
1Jiangxi Province Key Laboratory of Breast Diseases, Nanchang People's Hospital, Nanchang People's Hospital Affiliated of Nanchang Medical College, Nanchang, Jiangxi, People's Republic of China.
Background:
Precise detection of HER2-positive breast cancer is vital for targeted therapy. This study integrates HER2-specific DNA aptamers with fluorescent silica nanoparticles (FSNPs) to develop a targeted imaging probe.
Methods:
HER2 aptamer-conjugated FSNPs (HApt-FSNPs) were synthesized and characterized. Specificity was evaluated in HER2-positive/negative cells and tumor sections via flow cytometry and microscopy. Targeting efficacy and biodistribution were assessed in tumor-bearing mice through systemic injection and real-time fluorescence imaging. Photostability and biosafety were systematically examined.
Results:
HApt-FSNPs showed uniform size, excellent dispersity, and enhanced photostability. They selectively bound HER2-positive cells and tumor tissues, with binding effectively blocked by free aptamer. In vivo imaging revealed specific accumulation in HER2-positive tumors, peaking at 6 hours post-injection, with minimal off-target signals. The probe demonstrated good biocompatibility in vitro and in vivo.
Conclusion:
The HApt-FSNP platform enables specific detection and in vivo imaging of HER2-positive breast cancer, highlighting its potential for diagnostic and bioimaging applications.
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