Draft genome sequence of multidrug-resistant Escherichia coli strain ASK-30 from wastewater in northern India

Area of Science:

• Microbiology
• Genomics
• Environmental Science

Background:

• Wastewater serves as a reservoir for antibiotic-resistant bacteria.
• Emergence of multidrug-resistant (MDR) pathogens poses a significant public health threat.
• Understanding the genetic basis of MDR is crucial for effective control strategies.

Purpose of the Study:

• To perform draft genome sequencing of a multidrug-resistant Escherichia coli strain (ASK-30).
• To identify antibiotic resistance genes within the sequenced genome.
• To contribute genomic data on MDR bacteria from wastewater in India.

Main Methods:

• Isolation and identification of Escherichia coli strain ASK-30 from wastewater.
• Whole-genome sequencing (WGS) of the isolate.
• Bioinformatic analysis to identify antimicrobial resistance (AMR) genes.

Main Results:

• The draft genome sequence of Escherichia coli ASK-30 was successfully obtained.
• The genome harbors significant antibiotic resistance genes, including blaCTX-M-15 and blaTEM-1.
• The presence of these genes indicates resistance to critical antibiotic classes.

Conclusions:

• The genomic data provides insights into the genetic makeup of MDR E. coli in Indian wastewater.
• The identified resistance genes highlight the potential for spread of antimicrobial resistance (AMR).
• This study underscores the importance of wastewater surveillance for monitoring MDR bacteria.