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Liver Regeneration

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...

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Related Experiment Video

Updated: Mar 18, 2026

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

Published on: April 4, 2018

Paracrine Factor Local Gradient-Generating System for Engineering Perfusable Vascularized Hepatocyte Tissues with

Yen-Hsiang Huang¹, Tadahiro Yamashita^1,2, Ryo Sudo^1,2

¹School of Integrated Design Engineering, Graduate School of Science and Technology, Keio University, Yokohama, Japan.

Advanced Healthcare Materials

|March 16, 2026

Summary

This summary is machine-generated.

Engineered liver tissues face vascularization challenges. This study developed a system enabling microvessels to penetrate hepatocyte tissue, promoting regeneration and cell proliferation via perfusion.

Keywords:

fibroblasts gelatin cryogels microfluidic devices paracrine factors perfusable vascularized hepatocyte tissues perfusion‐induced hepatocyte proliferative activity primary hepatocytes

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Last Updated: Mar 18, 2026

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

Perfusable Vascular Network with a Tissue Model in a Microfluidic Device

Published on: April 4, 2018

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

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Published on: May 30, 2016

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Generation and Grafting of Tissue-engineered Vessels in a Mouse Model

Published on: March 18, 2015

Area of Science:

Biomedical Engineering
Regenerative Medicine
Hepatology

Background:

Donor organ shortages necessitate engineered liver tissues.
Vascular integration is a key challenge in liver tissue engineering.
Achieving perfusable microvessels within engineered tissues remains difficult.

Purpose of the Study:

To develop a system for creating vascularized, perfusable hepatocyte tissues.
To recapitulate perfusion-mediated proliferative activity of primary hepatocytes in vitro.
To overcome limitations in current liver tissue engineering vascularization strategies.

Main Methods:

A paracrine factor local gradient (PFLG)-generating system was developed.
Fibroblast-loaded cryogels were integrated with a microfluidic device.
Angiogenesis was directed prior to hepatocyte seeding to enable microvessel penetration.

Main Results:

Microvessels successfully penetrated the 3D hepatocyte tissue, mimicking in vivo architecture.
Engineered tissues showed enhanced hepatocyte polarity and functional bile canaliculi.
Perfusion culture significantly induced hepatocyte proliferation (increased Ki67-positive cells) without polarity loss.

Conclusions:

Perfusion-mediated cues are critical for inducing hepatocyte cell-cycle re-entry while maintaining polarity.
The developed platform supports vascularized, functional liver tissue engineering.
This modular system provides a foundation for scalable, transplantable liver tissues.