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Production of Xylooligosaccharides (XOSs) with Anti-inflammatory and Prebiotic Activities Using Recombinant
Nguyen Cao Cuong1,2, Dietmar Haltrich3, Apichat Boontawan4
1Molecular Biotechnology Laboratory, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Abstract:
The enzymatic production of xylooligosaccharides (XOSs) from xylan-rich agricultural byproducts has gained attention as a sustainable and environmentally friendly approach. In this work, two forms of recombinant Bacillus subtilis xylanases, MBP-BsXynA11 (a fusion of a maltose-binding protein with the xylanase) and BsXynA11-His (the xylanase with a 10xHis-tag), were investigated for their ability to convert xylan into value-added XOS. Both enzymes exhibited similar temperature and pH optima at 60 °C and 6.5, with specific activities of 7,900 and 4,800 U/nmol, respectively. However, MBP-BsXynA11 demonstrated higher yields upon recombinant expression, as well as greater specificity and thermostability compared to BsXynA11-His. Optimal hydrolysis of xylan yielded 53% and 51% XOS for MBP-BsXynA11 and BsXynA11-His, respectively. The resulting XOS were water-soluble, with over 90% of the products consisting of xylobiose, xylotriose, and xylotetraose, while xylose accounted for less than 0.5%. Finally, XOS generated from MBP-BsXynA11 demonstrated both prebiotic and anti-inflammatory activities in human monocytes.
