Structural insight into the CUB2 domain's role in enteropeptidase-mediated trypsinogen activation
Qiuyue Song1, Lisi Peng1, Xiaoli Yang1
1Department of Gastroenterology, Shanghai Institute of Pancreatic Diseases, Changhai Hospital, Navy/Second Military Medical University, Shanghai 200433, China.
Abstract:
Elucidating the structure and function of enteropeptidase (EP) is essential for advancing our understanding of its biological significance, particularly in regulating trypsinogen activation. Using cryo-EM and enzymatic activity, we uncovered the significance of the CUB2 domain in mediating the cleavage of macromolecular substrates. We identified crucial binding loops and key residues for EP's proteolytic function. The mutation E574A enhanced the proteolytic activity of EP, whereas the mutation N619A diminished its cleavage efficiency, highlighting the importance of surface-charged interactions in modulating EP's activity. A proteolytic cycle was proposed to deepen our understanding of the trypsinogen activation by EP. This work offers valuable insights into the molecular mechanisms underlying EP's interaction with its substrate, and opens up avenues for therapeutically modulating EP-mediated proteolysis.
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