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Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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A Self-Activating Cyclic Amplification Near-Infrared Sonoafterglow Probe for High-Contrast Imaging In Vivo.

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A new near-infrared sonoafterglow nanoprobe (3SCe-NP) significantly enhances afterglow intensity and half-life for ultrasensitive bioimaging. This self-activating probe overcomes limitations of current afterglow agents, enabling deep-tissue tumor imaging and monitoring of immunotherapy responses.

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Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Optical Imaging

Background:

  • Afterglow luminescence offers high signal-to-background ratio (SBR) for bioimaging but suffers from low brightness and short half-lives.
  • Existing probes often require preirradiation and have limited penetration depth, hindering in vivo applications.
  • Multicomponent systems face challenges in fabrication, reproducibility, and stability.

Purpose of the Study:

  • To develop a novel, self-activating, cyclic amplification near-infrared (NIR) sonoafterglow nanoprobe (3SCe-NP).
  • To overcome limitations of current afterglow probes, including brightness, half-life, and reliance on external light sources.
  • To enable ultrasensitive, deep-tissue bioimaging and monitoring of therapeutic responses.

Main Methods:

  • Engineered a bis(diarylethenyl)terthiophene scaffold for the 3SCe-NP, integrating sonosensitizer and NIR afterglow emitter functions.
  • Investigated the probe's performance, including afterglow intensity, half-life, and signal-to-background ratio (SBR) compared to MEH-PPV.
  • Studied the multireactive-site architecture's interaction with reactive oxygen species (ROS) and its self-amplification mechanism.

Main Results:

  • Achieved a ~270-fold enhancement in afterglow intensity and a 7-fold extension in half-life compared to MEH-PPV.
  • Demonstrated a self-activating, cyclic oxidation process for sustained ROS generation and amplified afterglow.
  • Successfully applied the nanoprobe for deep-tissue longitudinal tumor imaging and monitoring immunotherapy responses in vivo.

Conclusions:

  • The 3SCe-NP represents a significant advancement in afterglow bioimaging, offering enhanced brightness, stability, and deep-tissue penetration.
  • The probe's self-activating and rechargeable nature overcomes limitations of conventional afterglow agents.
  • This technology provides a versatile tool for ultrasensitive biomedical imaging and therapeutic response monitoring.