Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Light and Enzymatic Cooperative Response in Supramolecular Fibers: A Synergistic Strategy for Potential Drug Delivery Applications.

Biomacromolecules·2026

Same author

Ultrafast tyrosine-based cell membrane modification via diazonium salts: A new frontier for biomedical applications.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026

Same author

"<i>Visualize</i>, <i>Describe</i>, <i>Compare</i>"─Nanoinformatics Approaches for Material-Omics.

ACS nano·2026

Same author

Uncovering Subpopulations in Extracellular Vesicles via Multiplexed Spectral PAINT Super-Resolution Microscopy.

Nano letters·2026

Same author

Molecular determinants of TNFR1:TNFα binding and dynamics in a physiological membrane environment.

Current research in structural biology·2026

Same author

Subcellular glycan-mannose receptor binding kinetics correlate with myeloid cell function.

Nature communications·2025

Search research articles

Related Experiment Video

Updated: Mar 18, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Phenotyping by Molecular Mobility: Low-Affinity Reversible Probes Enable Characterizing and Classifying Cells

Roger Riera¹, Laurence W Fitzpatrick¹, Simon Huet²

¹Department of Biomedical Engineering, Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, De Zaale, Eindhoven 5612AZ, The Netherlands.

Journal of the American Chemical Society

|March 17, 2026

Summary

We developed a super-resolution method to analyze cell membrane receptor mobility for functional cell phenotyping. This technique uses novel probes to track receptors in live cells, revealing distinct mobility patterns that reflect cell type differences.

More Related Videos

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Published on: June 30, 2017

Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

Published on: October 21, 2021

Related Experiment Videos

Last Updated: Mar 18, 2026

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Discrimination and Characterization of Heterocellular Populations Using Quantitative Imaging Techniques

Published on: June 30, 2017

Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue

Published on: October 21, 2021

Area of Science:

Cell Biology
Biophysics
Molecular Imaging

Background:

Cell surface receptors are crucial for cellular functions and vary across cell types.
Understanding receptor behavior is key to differentiating cell functions and pathogenicity.
Existing methods often require genetic modification or labeling, perturbing native cell states.

Purpose of the Study:

To present a super-resolution method for functional cell phenotyping using single-molecule analysis of membrane receptor mobility.
To enable the study of endogenous receptors in unperturbed live cells.
To establish a workflow for cell phenotyping based on receptor mobility patterns.

Main Methods:

Development of nanofitin-based low-affinity probes for selective, reversible binding to target receptors.
Single-molecule tracking and analysis of membrane receptor mobility in live cells.
Integration with super-resolution microscopy (Point Accumulation for Imaging in Nanoscale Topography) and AI-driven classification.

Main Results:

Demonstrated that identical receptors exhibit distinct mobility patterns across different cell types.
Showcased the ability to detect, count, and track individual endogenous receptors without genetic modification or labeling.
Validated the method for functional phenotyping of cell populations based on receptor mobility.

Conclusions:

The presented super-resolution method provides a powerful tool for functional cell phenotyping.
Analysis of membrane receptor mobility offers insights into underlying physiological differences between cell types.
This approach allows for the study of endogenous biomolecules in their native cellular environment.