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Updated: Mar 18, 2026

Experimental Protocol for Manipulating Plant-induced Soil Heterogeneity
Published on: March 13, 2014
Heterogeneous responses of soil microbial communities and functions in coastal wetlands to long-term nitrogen
Mengyue Sun1, Mingcong Li1,2, Baohua Xie3,4,5
1College of Life Sciences, Shandong Agricultural University, Tai'an, China.
Abstract:
Global nitrogen (N) deposition, a major consequence of climate change, has profound impacts on soil microbes, yet comparative studies investigating the effects of different N types and levels on diverse soil microorganisms and their ecological functions remain scarce. Here, we conducted a 7-year simulated N deposition with multiple levels (Low, Medium, High) and multiple forms (NH4NO3, NH4Cl, KNO3), combining amplicon sequencing, QMEC chips and ¹⁵N isotope tracing to analyze their impacts on soil microbial communities of prokaryotes, fungi and Cercozoa, elemental cycling, and N transformation. The results showed that N forms dominated Cercozoa and prokaryote community structures (P < 0.05) but not fungi. High NH₄⁺-N deposition significantly reduced microbial resistance (P < 0.05), while NO₃--N deposition enhanced prokaryote-Cercozoa bipartite network stability and linearly increased community resistance (P < 0.05), supporting the ecological phenomenon of "Interdependence Leading to Enhanced Resilience". Elevated N deposition increased microbial diversity but inhibited key C / N cycling genes (e.g., pmoA, hzsB, nirK2, and nirS1) and N transformation (P < 0.05), raising CH₄ emission and soil N enrichment risks. In conclusion, this study provides scientific support for mitigating N deposition impacts and advancing environmental sustainability.
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