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Urinary Bladder

Urinary Bladder

The urinary bladder is a hollow, muscular sac that temporarily stores urine before it is expelled from the body. It can hold approximately 600 mL of urine prior to micturition. The bladder is retroperitoneal and located behind the pubic symphysis in the pelvic floor.
In males, the bladder is situated in front of the rectum, while in females, it is positioned anterior to the vagina and uterus. The bladder floor contains an inverted triangular area called the trigone, defined by the two ureteric...

Anatomy of the Genitourinary System II: Bladder and Urethra

Anatomy of the Genitourinary System II: Bladder and Urethra

The lower urinary system consists of the urinary bladder and urethra, which are essential in storing and expelling urine from the body. Together with the internal and external sphincters, these structures work together to regulate urination effectively.Anatomy of the BladderThe urinary bladder is a muscular, stretchable organ behind the pubic bone and in front of the rectum. In females, the bladder is positioned anterior to the vagina and inferior to the uterus, while in males, it is located...

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Related Experiment Video

Updated: May 10, 2026

Urinary Tract Infection in a Small Animal Model: Transurethral Catheterization of Male and Female Mice

Urinary Tract Infection in a Small Animal Model: Transurethral Catheterization of Male and Female Mice

Published on: December 1, 2017

Spatial transcriptomic map of the mouse urinary bladder.

Nives Matković¹, Andrea Gelemanović², Kristijana Popović¹

¹Laboratory for Cancer Research, School of Medicine, University of Split, Split, Croatia.

Scientific Reports

|March 17, 2026

Summary

This summary is machine-generated.

This study maps the mouse urinary bladder's gene expression using spatial transcriptomics. The findings reveal distinct cell types and gradual tissue layer transitions, enhancing our understanding of bladder biology.

Keywords:

lamina propria mouse urinary bladder smooth muscle spatial transcriptomics urothelium

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Last Updated: May 10, 2026

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Published on: December 1, 2017

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Published on: August 14, 2019

A Single Cell Dissociation Approach for Molecular Analysis of Urinary Bladder in the Mouse Following Spinal Cord Injury

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Published on: June 17, 2020

Area of Science:

Urology
Genomics
Spatial Biology

Background:

Understanding the spatial organization of the urinary bladder is crucial for diagnosing and treating bladder pathologies.
Traditional histological and single-cell RNA sequencing (scRNA-seq) methods have limitations in capturing tissue architecture and cell-cell interactions.

Purpose of the Study:

To create a high-resolution spatial transcriptomic map of the mouse urinary bladder.
To identify distinct cell populations and their spatial distribution within the bladder wall.
To reveal gene expression patterns and cellular transitions across different bladder compartments.

Main Methods:

Utilized Visium HD spatial transcriptomics technology on mouse urinary bladder tissue.
Analyzed 66,471 spatial bins and 9,364 genes after quality filtering.
Annotated cell clusters using canonical marker genes and integrated reference datasets (Tabula Muris, Mouse Cell Atlas).

Main Results:

Identified 13 distinct clusters corresponding to urothelium, lamina propria, and smooth muscle layers.
Observed gradual gene expression transitions across tissue layers, not sharp boundaries.
Differentiated basal, intermediate, and umbrella cells within the urothelium, noting potential mRNA polarization in umbrella cells.
Detected four distinct smooth muscle clusters, unlike the single cluster from non-spatial scRNA-seq.
Found lamina propria composed mainly of fibroblasts and immune cells.

Conclusions:

Spatial transcriptomics provides a powerful complement to histology and scRNA-seq for bladder research.
The generated map offers a valuable resource for studying mouse urinary bladder biology.
This study lays the groundwork for future investigations into bladder diseases and their spatial underpinnings.