Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

58.8K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.8K
CRISPR01:59

CRISPR

18.8K
18.8K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.5K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.5K
Transgenic Plants02:50

Transgenic Plants

9.1K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
9.1K
Plant Breeding and Biotechnology01:59

Plant Breeding and Biotechnology

22.1K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
22.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SNAP Transcription Factors Modulate Context-Dependent Senescence-Related Genes to Impact Soybean Yield and Seed Quality.

Plant physiology·2026
Same author

A GmWRKY20-GmbZIP9 transcriptional module synergistically activates GmANKTM21 to confer drought tolerance in soybean.

Plant physiology·2026
Same author

Paralogous LRR receptor kinases confer symbiosis specificity between arbuscular mycorrhizal and root nodule symbioses in Lotus japonicus.

The New phytologist·2026
Same author

Preferential induction of Dt2 gene in the absence of E1 function enhances semi-determinate stem growth in soybean.

Journal of experimental botany·2026
Same author

Multiplex gene editing drives revolution in crop breeding: overlaid editing of multiple genes and customization of complex traits.

Advanced biotechnology·2026
Same author

A flanking-nicks prime editor (FLICK-PE) system to boost prime editing in dicots.

Nature communications·2025
Same journal

Climate-driven niche filtering limits post-dispersal establishment and genomic introgression in a riverine shrub.

Journal of integrative plant biology·2026
Same journal

RING E3 ligase SNIPER8 functions redundantly with three close homologs to regulate the turnover of transcriptional co-repressor TPR1.

Journal of integrative plant biology·2026
Same journal

Duet between sugars and hormones: The molecular dialogue fine-tuning hypoxia acclimation in plants.

Journal of integrative plant biology·2026
Same journal

Engineering glyphosate-resistant watermelon by prime editing.

Journal of integrative plant biology·2026
Same journal

ABA orchestrates the expression of SWEET16 for sugar accumulation via phosphorylation and transcriptional mechanisms in citrus.

Journal of integrative plant biology·2026
Same journal

Cross-kingdom RNA decoy redefines fungal virulence strategies.

Journal of integrative plant biology·2026
See all related articles

Related Experiment Video

Updated: Mar 18, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.6K

Developing a robust multiplex CRISPR/Cas12i3-5M system for trait stacking in soybean.

Wenxin Lin1, Haijie Wu2, Huaqin Kuang3

  • 1Sanya Institute of China Agricultural University, Sanya, 572000, China.

Journal of Integrative Plant Biology
|March 17, 2026
PubMed
Summary
This summary is machine-generated.

A novel CRISPR gene editing tool efficiently modified 13 sites in soybean, creating varieties with healthier oils and reduced beany flavor.

More Related Videos

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
12:59

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Published on: April 30, 2016

18.8K
A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
09:22

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

26.7K

Related Experiment Videos

Last Updated: Mar 18, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.6K
High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots
12:59

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Published on: April 30, 2016

18.8K
A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
09:22

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses

Published on: January 25, 2018

26.7K

Area of Science:

  • Agricultural biotechnology
  • Plant genetics
  • Molecular biology

Background:

  • Soybean oil quality is crucial for food processing and health.
  • Traditional breeding methods for improving soybean oil profiles are time-consuming.
  • Developing efficient gene editing tools is essential for crop improvement.

Purpose of the Study:

  • To develop and apply a high-efficiency multiplex gene editing system for soybean.
  • To generate soybean germplasm with enhanced oleic acid content.
  • To eliminate the characteristic beany flavor in soybean.

Main Methods:

  • Utilized the CRISPR-Cas12i3-5M system for multiplex gene editing.
  • Targeted 13 specific genomic loci simultaneously in soybean.
  • Evaluated the resulting soybean germplasm for oil composition and sensory attributes.

Main Results:

  • Achieved simultaneous editing at 13 target sites with high efficiency.
  • Successfully generated soybean lines with significantly increased oleic acid content.
  • Developed soybean germplasm exhibiting a complete absence of beany flavor.

Conclusions:

  • The CRISPR-Cas12i3-5M system is a powerful tool for rapid soybean improvement.
  • This technology enables the development of healthier and more palatable soybean varieties.
  • Multiplex gene editing offers a promising avenue for accelerating crop trait development.