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Updated: Mar 24, 2026

Analytical Techniques for Assaying Nitric Oxide Bioactivity
Published on: June 18, 2012
Chemical constituents from Atractylodes japonica and their inhibitory effect on NO production
Jiaqi Liu1, Jinyao Wang1, Peng Yu1
1Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education; Molecular Medicine Research Center, College of Pharmacy, Yanbian University, Yanji, Jilin Province, China.
Abstract:
A new sesquiterpenoid, 7β-hydroxy-cnid-4(15),11(13)-dien-8-one (1) and a new polyacetylene, (4E,6Z,12E)-tetradecatriene-8,10-diyn-1-yl acetate (2), along with twenty known analogs (3-22), were obtained from Atractylodes japonica. Their chemical structures were established by spectroscopic techniques. All the isolates were evaluated for their inhibitory activity against NO production in LPS-induced RAW 264.7 cells, and compounds 2-7, 9-16, 18-22 exhibited inhibitory activities with IC50 values ranging from 2.39 ± 0.54 to 46.51 ± 0.87 μM.
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