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Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Physics-informed deep learning framework for wavefront sensing via optical beam pattern analysis.

Tengfei Chai, Xiaoyun Liu, Hongwei Wang

    Journal of the Optical Society of America. A, Optics, Image Science, and Vision
    |March 17, 2026
    PubMed
    Summary
    This summary is machine-generated.

    We introduce PIRNet, a physics-informed deep learning model for efficient wavefront sensing. This novel framework accurately estimates optical aberrations, improving adaptive optics and optical communication systems.

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    Area of Science:

    • Optical Engineering
    • Deep Learning Applications
    • Wavefront Sensing

    Background:

    • Non-interferometric wavefront sensing is crucial for optical systems.
    • Existing methods often lack accuracy or efficiency.
    • Aberrations like spherical aberration, coma, and astigmatism degrade optical performance.

    Purpose of the Study:

    • To develop a lightweight, physics-informed deep learning framework (PIRNet) for wavefront sensing.
    • To enable simultaneous estimation of multiple aberrations from single-shot intensity patterns.
    • To enhance accuracy and generalization in optical beam expansion systems.

    Main Methods:

    • Proposed PIRNet, a deep learning framework integrating physics-informed loss.
    • Generated a large dataset using vortex beam propagation simulations and ABCD matrix method.
    • Implemented a physics-consistency loss based on optical propagation models.
    • Utilized a staged training strategy and learnable uncertainty weighting.

    Main Results:

    • PIRNet accurately estimates spherical aberration, coma, and astigmatism.
    • The physics-consistency loss improves physical plausibility and model generalization.
    • PIRNet outperformed ResNet and Xception models in comparative experiments.
    • Performance was robust across varying noise levels and cropping ratios.

    Conclusions:

    • PIRNet offers a promising, accurate, and generalizable approach for wavefront characterization.
    • The integration of physical priors enhances deep learning model performance in optics.
    • PIRNet has potential applications in adaptive optics and free-space optical communication.