Related Experiment Video
Updated: Mar 19, 2026

Direct Imaging of Laser-driven Ultrafast Molecular Rotation
Published on: February 4, 2017
Tailored fractional vortex beams for incoherent imaging and deconvolution
Abstract:
Imaging under spatially incoherent illumination remains a key challenge due to resolution loss from diffraction-limited point-spread functions (PSFs). We investigate engineered PSFs generated by two fractional vortex phase masks, the anomalous multiramp phase plate, and the elliptical fractional vortex phase plate, applied here for the first time, to our knowledge, in incoherent imaging. These phase domain-coded apertures introduce controllable phase discontinuities, singularity distributions, and anisotropic diffraction features, enabling flexible PSF shaping through fractional topological charge, ramp geometry, and ellipticity. We analyze how parameter variations transform PSF structures, from compact spiral lobes to extended asymmetric patterns, and discuss their implications for spatial encoding, directional selectivity, and depth discrimination. To overcome the reconstruction challenges posed by such PSFs, we propose a frequency regularized nonlinear reconstruction (FR-NLR) algorithm that incorporates spectral priors for enhanced stability. Quantitative and qualitative evaluations show that FR-NLR consistently outperforms the nonlinear reconstruction (NLR), Lucy-Richardson-Rosen algorithm, and Wiener deconvolution, achieving compact, high-fidelity, robust, and scan-free incoherent imaging.
Related Concept Videos
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Deconvolution
Deconvolution involves several mathematical techniques to derive the impulse response. One common approach is polynomial division. In this method, the input and output sequences are treated as coefficients of...
Confocal Fluorescence Microscopy
Electron Microscope Tomography and Single-particle Reconstruction
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

