Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

2.9K
Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
2.9K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.8K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.8K
Therapeutic Drug Monitoring: Drug Analysis Methods01:26

Therapeutic Drug Monitoring: Drug Analysis Methods

276
Therapeutic Drug Monitoring (TDM) is a clinical practice that measures specific drug levels in a patient's blood or body tissues to tailor drug therapy effectively. This monitoring is critical for managing drugs with narrow therapeutic indices like digoxin and phenytoin, ensuring they are both safe and effective. For instance, monitoring theophylline levels in asthma patients involves precision and sensitivity to adjust doses according to individual responses to therapy, ensuring efficacy and...
276

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A fatty acid amide activates myeloid cells and improves neurovascular outcomes in retinal degeneration.

Nature neuroscience·2026
Same author

Decoupling of global metabolic flux and proteome partitioning in bacteria.

Science (New York, N.Y.)·2026
Same author

Maximizing Lipidome Coverage of Mouse Liver Following the IV Administration of Gefitinib by Combining Both UHPLC-MS-Based Untargeted and Targeted Lipidomics.

Journal of proteome research·2026
Same author

Expression of Axonal Transport Proteins in Dopaminergic Neurons of the Substantia Nigra in Mouse Models of Preclinical and Clinical Stages of Parkinson's Disease.

International journal of molecular sciences·2026
Same author

Development and Validation of a Streamlined Workflow for Proteomic Analysis of Proteins and Post-translational Modifications from Dried Blood.

Journal of proteome research·2026
Same author

Association of acylcarnitine species and diabetes incidence in a population-based apparently healthy cohort.

Cardiovascular diabetology. Endocrinology reports·2026
Same journal

AutoPELSA: An Automated Sample Preparation System for Proteome-Wide Identification of Target Proteins of Diverse Ligands.

Analytical chemistry·2026
Same journal

Keeping Reviews Meaningful in the Era of AI.

Analytical chemistry·2026
Same journal

Assessment of Papillary Thyroid Carcinoma by Profiling Multiple Matrix Metalloproteinase Activities Using a Machine Learning-Assisted Peptide Microarray Sensing Platform.

Analytical chemistry·2026
Same journal

Dual-Functional Ratio Photoelectrochemical Biosensing Platform for Photothermal Simultaneous Sterilization and Lipopolysaccharides-Mediated Detection of <i>Escherichia coli</i> O157:H7.

Analytical chemistry·2026
Same journal

Composite Peak Scoring for Improved Charged Aerosol Detector-Based Universal Quantification of Drug Libraries.

Analytical chemistry·2026
Same journal

UV-Triggered Chemiluminescence as a Bulk Surrogate Indicator for Microplastic Contamination in Aquatic Matrices.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Mar 19, 2026

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

5.1K

Untargeted Multiple Reaction Monitoring.

Winnie Uritboonthai1, Aries Aisporna1, Linh Hoang1

  • 1Scripps Center for Metabolomics and Mass Spectrometry, The Scripps Research Institute, La Jolla, California 92037, United States.

Analytical Chemistry
|March 17, 2026
PubMed
Summary
This summary is machine-generated.

We developed untargeted/micro/universal multiple reaction monitoring (uMRM) to automate the creation of quantitative mass spectrometry methods from discovery data. This workflow converts untargeted LC-MS/MS data into scheduled MRM transitions for robust quantification.

More Related Videos

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
11:09

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

10.7K
Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

17.7K

Related Experiment Videos

Last Updated: Mar 19, 2026

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue
11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

5.1K
An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
11:09

An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

Published on: April 17, 2018

10.7K
Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification
09:04

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

17.7K

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Mass Spectrometry

Background:

  • Multiple reaction monitoring (MRM) offers sensitive and robust quantification but requires predefined transitions, limiting its application in discovery studies.
  • Traditional MRM workflows necessitate extensive optimization and specific standards, posing challenges for large-scale or exploratory research.

Purpose of the Study:

  • To introduce untargeted/micro/universal multiple reaction monitoring (uMRM), a novel workflow for automated generation of quantitative MRM methods.
  • To enable the conversion of high-resolution untargeted liquid chromatography-mass spectrometry/MS (LC-MS/MS) data into scheduled triple-quadrupole MRM transitions.
  • To provide a reproducible method for linking untargeted LC-MS/MS acquisition with targeted quantitation without authentic standards.

Main Methods:

  • Utilized pooled-sample LC-MS and stepped-energy data-dependent acquisition (DDA) MS/MS for precursor and fragment ion information capture.
  • Implemented automated deisotoping, in-source fragment filtering, and spline-based modeling of collision energy-dependent fragmentation.
  • Scheduled transitions using retention times from pooled samples for deployment on triple-quadrupole instruments.

Main Results:

  • Successfully generated quantitative MRM methods from untargeted discovery data across diverse biological matrices (urine, brain tissue, cultured cells).
  • Demonstrated strong agreement between uMRM-derived and experimentally optimized collision energies across seven triple-quadrupole platforms.
  • Validated the ability to deploy MRM transitions without nonlinear retention-time alignment or authentic standards.

Conclusions:

  • uMRM provides a reproducible and automated approach to convert untargeted LC-MS/MS discovery data into quantitative MRM methods.
  • This workflow significantly expands the utility of MRM for discovery-driven research by simplifying method development.
  • uMRM facilitates the transition from broad-scale data acquisition to targeted, high-sensitivity quantitation.