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Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells
Published on: June 5, 2020
Antiviral and Mutagenic Effects of APOBEC Deaminases A3C, A3D, and A3H in a Hepatitis B Virus Model
Ivan V Karandashov1,2, Sergey A Brezgin3,2, Natalia I Ponomareva4
1Laboratory of Genetic Technologies in Drug Development, E. I. Martsinovsky Institute of Medical Parasitology, Tropical and Transmissible Diseases, Sechenov University, Moscow, 119435, Russia. ivan.karandashov@gmail.com.
Abstract:
Hepatitis B virus (HBV) infects human hepatocytes, causing acute or chronic liver infection. Chronic HBV infection leads to progressive liver damage, potentially resulting in cirrhosis or hepatocellular carcinoma. One promising antiviral strategy involves activating cytidine deaminases of the APOBEC/AID family, which could induce mutational degradation of HBV. Using a CRISPRa-based transcriptional activation system with modified sgRNAs, we investigated antiviral and oncogenic effects of the activating genes encoding APOBEC3C, APOBEC3D, and APOBEC3H.
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