Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulated Protein Degradation02:58

Regulated Protein Degradation

9.2K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
9.2K
Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

9.9K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
9.9K
Antiepileptic Drugs: Modulators of Neurotransmitter Release Mediated by SV2A Protein01:20

Antiepileptic Drugs: Modulators of Neurotransmitter Release Mediated by SV2A Protein

1.1K
Antiepileptic drugs, such as levetiracetam (Keppra) and brivaracetam (Briviact), have emerged as crucial tools in managing epilepsy. These medications exert their therapeutic effects by targeting the synaptic vesicle protein SV2A, a transmembrane glycoprotein primarily found in the brain.
SV2A is a transmembrane glycoprotein located predominantly in the brain, modulating the release of neurotransmitters for neuronal communication. Both levetiracetam and brivaracetam exhibit a high affinity for...
1.1K
Drugs Affecting Neurotransmitter Release or Uptake01:21

Drugs Affecting Neurotransmitter Release or Uptake

1.8K
Certain drugs can affect how neurotransmitters called catecholamines, are released or taken back up in the adrenergic neuron. They can have different effects on the body's sympathetic transmission. Reserpine, a natural compound found in the Rauwolfia shrub, blocks a transporter called vesicular monoamine transporter (VMAT), which leads to a buildup of catecholamines in the cell and reduces sympathetic transmission. Another drug called guanethidine works in multiple ways, including blocking...
1.8K
The Proteasome01:13

The Proteasome

1.9K
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
1.9K
The Proteasome02:18

The Proteasome

10.4K
Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
10.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In a room or on Zoom? Trying out an online research course for people with intellectual disabilities.

Journal of intellectual disabilities : JOID·2026
Same author

Monoallelic and biallelic KDM5A variants identified in patients with autism spectrum disorder.

HGG advances·2026
Same author

Creativity as a core competency in medical education: the art of medicine.

Medical humanities·2026
Same author

Traumatic stress intervention research for the South Asian American diaspora: A methodological protocol article.

Psychological trauma : theory, research, practice and policy·2026
Same author

Benchmarking and optimizing Perturb-seq in differentiating human pluripotent stem cells.

Stem cell reports·2025
Same author

Educational Intervention to Build Concepts on Drug-Related Adverse Events, their Monitoring and Reporting among Nursing Students: A Multicentric Longitudinal Study in North India.

Indian journal of community medicine : official publication of Indian Association of Preventive & Social Medicine·2025

Related Experiment Video

Updated: Mar 19, 2026

A Scalable, Cell-Based Method for the Functional Assessment of Ube3a Variants
06:35

A Scalable, Cell-Based Method for the Functional Assessment of Ube3a Variants

Published on: October 10, 2022

2.5K

The E3 Ubiquitin Ligase UBE3B Regulates Synaptic Development and Cortical Network Activity.

Shayal Vashisth1, Aleya Shedd1, Ariel Aiken2

  • 1Department of Neuroscience, University of Texas Southwestern Medical Center, Dallas, Texas, USA.

Autism Research : Official Journal of the International Society for Autism Research
|March 17, 2026
PubMed
Summary

Loss of UBE3B causes severe neurodevelopmental deficits in mice, impacting social behavior, learning, and motor skills. This highlights UBE3B

More Related Videos

In Vitro Analysis of E3 Ubiquitin Ligase Function
06:06

In Vitro Analysis of E3 Ubiquitin Ligase Function

Published on: May 14, 2021

6.1K
In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
11:36

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones

Published on: July 25, 2019

11.6K

Related Experiment Videos

Last Updated: Mar 19, 2026

A Scalable, Cell-Based Method for the Functional Assessment of Ube3a Variants
06:35

A Scalable, Cell-Based Method for the Functional Assessment of Ube3a Variants

Published on: October 10, 2022

2.5K
In Vitro Analysis of E3 Ubiquitin Ligase Function
06:06

In Vitro Analysis of E3 Ubiquitin Ligase Function

Published on: May 14, 2021

6.1K
In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
11:36

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones

Published on: July 25, 2019

11.6K

Area of Science:

  • Neuroscience
  • Genetics
  • Molecular Biology

Background:

  • Autism spectrum disorder (ASD) is a neurodevelopmental disorder linked to impaired communication and social interaction.
  • Pathogenic mutations in UBE3B cause neurodevelopmental diseases, including ASD.
  • The ubiquitin-proteasome system (UPS) is crucial for neurodevelopment, with UPS gene mutations implicated in ASD.

Purpose of the Study:

  • To investigate the brain function of UBE3B and its role in neurodevelopmental abnormalities.
  • To generate and evaluate a central nervous system-specific conditional Ube3b knockout (cKO) mouse model.

Main Methods:

  • Generated a central nervous system-specific conditional Ube3b knockout (cKO) mouse model.
  • Evaluated neurobehavioral phenotypes, including vocalization, social behavior, learning, memory, and motor skills.
  • Assessed in vivo neuronal phenotypes, proteome, and ubiquitome of neural stem cells.

Main Results:

  • Ube3b cKO mice displayed severe deficits in vocalization, social behavior, learning, memory, and motor skills.
  • Neuronal analyses revealed defects in dendritic morphogenesis, reduced excitatory synapse density, and altered cortical circuit activity.
  • Proteomic analysis identified 116 proteins with altered levels and ubiquitination in Ube3b-deficient neural stem cells, enriched for synaptic functions.

Conclusions:

  • UBE3B plays a critical role in regulating social, cognitive, and motor functions.
  • UBE3B is essential for neuronal morphogenesis and activity by modulating the synaptic proteome.
  • Disruption of UBE3B function contributes to neurodevelopmental abnormalities relevant to ASD.