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Gene Regulation During Sporulation

Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
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Basidiomycota is a diverse phylum of fungi that includes ecologically significant decomposers such as white rot fungi, symbionts like mycorrhizal fungi, plant pathogens such as rusts and smuts, and edible species like Agaricus bisporus (the common button mushroom). These fungi play crucial roles in nutrient cycling, symbiotic relationships, and even human health. Their defining feature is the basidium, a microscopic club-shaped structure responsible for producing basidiospores.Fruiting Bodies...

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Transcriptomic insights into fruiting body malformations in Lentinula edodes.

Tong Lin1,2,3, Juan Du1, Shipeng Li1

  • 1College of Life Science, Langfang Normal University, Langfang, 065000, Hebei, China.

Applied Microbiology and Biotechnology
|March 18, 2026
PubMed
Summary
This summary is machine-generated.

Shiitake mushroom malformations stem from specific gene expression changes. Two key genes are consistently altered across all deformities, offering targets for improving shiitake cultivation and yield.

Keywords:
ShiitakeMalformationMetabolic pathwaysTranscriptome analysisWeighted gene co-expression network analysis

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Area of Science:

  • Mycology
  • Molecular Biology
  • Genomics

Background:

  • Lentinula edodes (shiitake mushroom) cultivation faces economic losses due to malformed fruiting bodies.
  • Understanding the molecular basis of these deformities is crucial for improving crop yield.

Purpose of the Study:

  • To identify molecular differences underlying four types of shiitake mushroom malformations.
  • To pinpoint conserved genetic factors contributing to abnormal development.

Main Methods:

  • Comparative transcriptome analysis of four malformation types (H, P, T, I) and normal controls.
  • Differential gene expression analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis.
  • Weighted gene co-expression network analysis.

Main Results:

  • Deformity-specific transcriptional signatures were identified, with significant differentially expressed genes (DEGs) in T and I phenotypes.
  • Two DEGs (LENED_000547 and LENED_004546) were consistently found across all malformed types.
  • Functional enrichment revealed dysregulation in genes related to cellular structure, metabolism, and stress response.

Conclusions:

  • Intrinsic transcriptomic dysregulation is a primary driver of L. edodes malformations.
  • Conserved DEGs provide potential targets for precision breeding and cultivation management.
  • Findings guide strategies to enhance shiitake mushroom quality and yield.