Jove
Visualize
Contact Us

Related Concept Videos

Protein Folding Quality Check in the RER01:29

Protein Folding Quality Check in the RER

5.4K
ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
5.4K
Improving Translational Accuracy02:07

Improving Translational Accuracy

15.4K
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
15.4K
Bacterial Protein Maturation01:26

Bacterial Protein Maturation

674
Bacterial protein maturation is a tightly regulated process that ensures newly synthesized polypeptides achieve correct functional conformations. This maturation involves a series of modifications, folding events, and quality control steps, often assisted by specialized chaperone proteins.N-Terminal ModificationsThe maturation of bacterial polypeptides begins cotranslationally as the polypeptide exits the ribosome. The first amino acid, N-formylmethionine (fMet), is typically modified at the...
674
Translational Regulation01:29

Translational Regulation

763
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
763
Ribosomes01:27

Ribosomes

11.6K
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
11.6K
Ribosomes01:27

Ribosomes

80.2K
Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...
80.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

cGAS-IFN-I responses by extracting nuclear DNA from dying cells via nucleocytosis.

Nature communications·2026
Same author

Crosstalk between the ribosome quality control-associated E3 ubiquitin ligases LTN1 and RNF10.

FEBS letters·2025
Same author

Collision-induced ribosome degradation driven by ribosome competition and translational perturbations.

Nature communications·2025
Same author

Polyubiquitin architecture editing on collided ribosomes maintains persistent RQC activity.

The EMBO journal·2025
Same author

Herpes simplex virus 1 evades APOBEC1-mediated immunity via its uracil-DNA glycosylase in mice.

Nature microbiology·2025
Same author

Structure of a Gcn2 dimer in complex with the large 60S ribosomal subunit.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same journal

Promoter reinforcement supports transcriptional resilience in drug-resistant cancer.

Nature structural & molecular biology·2026
Same journal

Publisher Correction: Interplay between cohesin and RNA polymerase II in regulating chromatin interactions and gene transcription.

Nature structural & molecular biology·2026
Same journal

An asymmetric non-canonical nucleosome shapes the directionality of transcription outcomes.

Nature structural & molecular biology·2026
Same journal

Structural insights into neurokinin 2 receptor selectivity hold implications for obesity therapeutics.

Nature structural & molecular biology·2026
Same journal

Genome-wide absolute quantification of chromatin looping.

Nature structural & molecular biology·2026
Same journal

Putting numbers on chromatin looping.

Nature structural & molecular biology·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Mar 19, 2026

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
06:58

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

Published on: October 7, 2021

3.1K

Ribosome-associated quality control and related mechanisms.

Toshifumi Inada1

  • 1Division of RNA and gene regulation, Institute of Medical Science, The University of Tokyo, Minato-Ku, Japan. toshiinada@ims.u-tokyo.ac.jp.

Nature Structural & Molecular Biology
|March 18, 2026
PubMed
Summary
This summary is machine-generated.

Ribosome-associated quality control (RQC) detects and resolves collided ribosomes to prevent cellular stress. This review details RQC mechanisms, disease links, and its role in protecting cells from protein homeostasis collapse.

More Related Videos

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
12:05

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Published on: August 7, 2021

9.5K
Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
12:57

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Published on: December 21, 2017

12.0K

Related Experiment Videos

Last Updated: Mar 19, 2026

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
06:58

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

Published on: October 7, 2021

3.1K
RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
12:05

RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

Published on: August 7, 2021

9.5K
Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling
12:57

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Published on: December 21, 2017

12.0K

Area of Science:

  • Molecular Biology
  • Cellular Biology
  • Biochemistry

Background:

  • Ribosome collisions during translation pose a threat to cellular function.
  • Nascent polypeptide chains require proper handling when ribosomes stall.
  • Existing quality control mechanisms are crucial for maintaining proteostasis.

Purpose of the Study:

  • To review the mechanisms of Ribosome-associated quality control (RQC).
  • To explore the crosstalk between RQC pathways and their implications in diseases.
  • To highlight RQC's role as a critical cellular defense system.

Main Methods:

  • Literature review of RQC mechanisms.
  • Analysis of RQC pathway crosstalk.
  • Examination of disease-associated RQC dysfunction.

Main Results:

  • RQC identifies and resolves collided ribosomes.
  • RQC pathways triage aberrant nascent chains.
  • RQC acts as a rapid cellular response to translational stress.

Conclusions:

  • RQC is essential for preventing global stress responses.
  • Dysfunctional RQC contributes to proteostasis collapse and disease.
  • RQC serves as a vital 'first responder' in cellular quality control.