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Colonic inflammatory response in a D-galactose-induced aging model: elevated IL-1β expression
Cherry Azaria1,2, Rina Susilowati3, Yustina Andwi Ari Sumiwi3
1Doctoral Program Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Jalan Farmako, Sekip Utara, Yogyakarta, 55281, Indonesia.
Journal of Molecular Histology
|March 18, 2026
Summary
D-galactose (D-Gal) induces aging in rats, leading to colonic inflammation. This aging model shows increased Interleukin-1 beta (IL-1β) expression, indicating cellular senescence and inflammation in the gut.
Area of Science:
- Gerontology
- Gastroenterology
- Molecular Biology
Background:
- Aging involves cellular changes like cell cycle arrest and senescence-associated secretory phenotype (SASP).
- D-galactose (D-Gal) is a chemical inducer used to create aging animal models.
- The impact of D-Gal on the colon and its aging markers is not well understood.
Purpose of the Study:
- To investigate the effects of D-Gal induced aging on the colon in Sprague-Dawley rats.
- To analyze the expression of key aging and inflammation markers in the colons of D-Gal treated rats.
Main Methods:
- Twelve-week-old male Sprague-Dawley rats were divided into control and D-Gal groups.
- D-Gal (100 mg/kg/day) was administered for 6 weeks.
- Gene expression (p53, Cdkn1a, Il-6, Il-1β, Lmnb1) was measured using RT-qPCR; histological analysis was performed.
Main Results:
- D-Gal administration resulted in inflammatory cell infiltration in the colon.
- Interleukin-1 beta (IL-1β) expression and immunohistochemical scores were significantly elevated in the D-Gal group.
- No significant changes were observed in p53, Il-6, Cdkn1a, or Lmnb1 gene expression.
Conclusions:
- D-galactose administration induces colonic inflammation in aging rat models.
- Elevated IL-1β expression suggests SASP activation and pro-inflammatory signaling.
- D-Gal treatment promotes potential cellular senescence in the colon.
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