Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inflammatory Bowel Disease II: Ulcerative Colitis01:20

Inflammatory Bowel Disease II: Ulcerative Colitis

Ulcerative colitis is a chronic inflammatory disorder of the colon characterized by continuous mucosal inflammation that typically begins in the rectum and extends proximally in a uniform pattern. Its pathogenesis involves a complex interplay of genetic predisposition, immune dysregulation, and environmental influences. These factors converge to impair the colon’s epithelial defenses and promote an exaggerated inflammatory response against luminal contents.Breakdown of the Mucosal BarrierA...
Inflammatory Bowel Disease III: Crohn's Disease01:25

Inflammatory Bowel Disease III: Crohn's Disease

Crohn’s disease is a chronic, relapsing form of inflammatory bowel disease characterized by segmental, transmural inflammation that can affect any part of the gastrointestinal tract. Its pathogenesis arises from a combination of genetic susceptibility, environmental exposures, epithelial barrier dysfunction, and immune dysregulation. Together, these factors lead to an exaggerated immune response against components of the gut microbiome.Genetic and Environmental InfluencesMultiple genetic...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Upregulated Klotho expression reflects a potential compensatory renal response in Trimethyltin-exposed rats.

Medical molecular morphology·2026
Same author

Stereological analysis of spleen alterations in streptozotocin-nicotinamide-induced diabetic rats.

Biotechnic & histochemistry : official publication of the Biological Stain Commission·2025
Same author

Inflammatory and morphological changes in the colon reflect early aging induced by D-galactose in rats.

Scientific reports·2025
Same author

TNF-α Inhibition Modulates CX3CR1, Pyroptosis-related Genes, and BDNF to Alleviate Anxiety-like Behavior in Type 2 Diabetic Rats.

Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology·2025
Same author

Salivary MicroRNAs as Potential Noninvasive Biomarkers for the Diagnosis of Nasopharyngeal Carcinoma: Protocol for a Scoping Review.

JMIR research protocols·2025
Same author

Targeting immune cells in tumor microenvironment in triple negative breast cancer therapy: future perspective to overcome doxorubicin resistance and toxicity.

Medical oncology (Northwood, London, England)·2025

Related Experiment Video

Updated: May 11, 2026

Investigating Intestinal Inflammation in DSS-induced Model of IBD
08:43

Investigating Intestinal Inflammation in DSS-induced Model of IBD

Published on: February 1, 2012

69.2K

Colonic inflammatory response in a D-galactose-induced aging model: elevated IL-1β expression.

Cherry Azaria1,2, Rina Susilowati3, Yustina Andwi Ari Sumiwi3

  • 1Doctoral Program Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Jalan Farmako, Sekip Utara, Yogyakarta, 55281, Indonesia.

Journal of Molecular Histology
|March 18, 2026
PubMed
Summary

D-galactose (D-Gal) induces aging in rats, leading to colonic inflammation. This aging model shows increased Interleukin-1 beta (IL-1β) expression, indicating cellular senescence and inflammation in the gut.

Keywords:
Aging-rat modelColonD-galactoseGene expressionInflammation

More Related Videos

DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat
09:04

DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat

Published on: February 27, 2014

49.3K
Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids
06:31

Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids

Published on: June 2, 2023

1.6K

Related Experiment Videos

Last Updated: May 11, 2026

Investigating Intestinal Inflammation in DSS-induced Model of IBD
08:43

Investigating Intestinal Inflammation in DSS-induced Model of IBD

Published on: February 1, 2012

69.2K
DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat
09:04

DNBS/TNBS Colitis Models: Providing Insights Into Inflammatory Bowel Disease and Effects of Dietary Fat

Published on: February 27, 2014

49.3K
Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids
06:31

Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids

Published on: June 2, 2023

1.6K

Area of Science:

  • Gerontology
  • Gastroenterology
  • Molecular Biology

Background:

  • Aging involves cellular changes like cell cycle arrest and senescence-associated secretory phenotype (SASP).
  • D-galactose (D-Gal) is a chemical inducer used to create aging animal models.
  • The impact of D-Gal on the colon and its aging markers is not well understood.

Purpose of the Study:

  • To investigate the effects of D-Gal induced aging on the colon in Sprague-Dawley rats.
  • To analyze the expression of key aging and inflammation markers in the colons of D-Gal treated rats.

Main Methods:

  • Twelve-week-old male Sprague-Dawley rats were divided into control and D-Gal groups.
  • D-Gal (100 mg/kg/day) was administered for 6 weeks.
  • Gene expression (p53, Cdkn1a, Il-6, Il-1β, Lmnb1) was measured using RT-qPCR; histological analysis was performed.

Main Results:

  • D-Gal administration resulted in inflammatory cell infiltration in the colon.
  • Interleukin-1 beta (IL-1β) expression and immunohistochemical scores were significantly elevated in the D-Gal group.
  • No significant changes were observed in p53, Il-6, Cdkn1a, or Lmnb1 gene expression.

Conclusions:

  • D-galactose administration induces colonic inflammation in aging rat models.
  • Elevated IL-1β expression suggests SASP activation and pro-inflammatory signaling.
  • D-Gal treatment promotes potential cellular senescence in the colon.