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    We developed a synchronized three-color fiber laser system for advanced vibrational microscopy. This system enables simultaneous broad spectral acquisition for Stimulated Raman Scattering (SRS) microscopy.

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    Area of Science:

    • Laser Physics
    • Optical Microscopy
    • Spectroscopy

    Background:

    • Mode-locked lasers are crucial for advanced microscopy techniques.
    • Broadband coherent Raman techniques like Stimulated Raman Scattering (SRS) require synchronized multi-color lasers.
    • Existing systems may lack the necessary synchronization or spectral range.

    Purpose of the Study:

    • To realize a synchronized three-color all-polarization-maintaining (PM) fiber laser system.
    • To enable multiplex SRS microscopy for simultaneous broad spectral acquisition.
    • To characterize the synchronization mechanism and laser relative intensity noise.

    Main Methods:

    • Utilized Er-, Yb-, and Nd-doped fiber lasers.
    • Implemented passive synchronization via cross-phase modulation in common PM-fibers.
    • Operated the system in a mode-locking regime at 1.56, 1.03, and 0.92 μm.

    Main Results:

    • Achieved passively synchronized three-color laser emission.
    • Demonstrated temporal pulse jitter below 400 fs over 1 s integration time.
    • Characterized synchronization and relative intensity noise.

    Conclusions:

    • The developed three-color laser system is core technology for multiplex SRS microscopy.
    • Enables simultaneous fast acquisition of broad spectra in C-H stretching and fingerprint regions.
    • Advances vibrational microscopy capabilities with enhanced spectral information.