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    Area of Science:

    • Biophotonics
    • Optical characterization
    • Turbid media analysis

    Background:

    • Separating absorption and scattering in turbid media is a significant challenge in optical characterization.
    • Existing methods often struggle to independently quantify these optical properties.

    Purpose of the Study:

    • To develop and validate a non-contact method for estimating the absorption coefficient (µa) using phase analysis.
    • To extend the iterative multi-plane optical properties extraction (IMOPE) technique for absorption assessment.

    Main Methods:

    • Applied a phase-analysis framework, originally for reduced scattering coefficient (µs') extraction, to estimate µa.
    • Extended the diffuse-reflectance (DR) model to include sensitivity to µa variations.
    • Validated using Monte Carlo simulations and TiO2-based phantoms with controlled optical properties.

    Main Results:

    • Demonstrated opposing trends for µa and µs' increments within the phase-analysis framework.
    • Experimentally validated the phase-based µa extraction scheme with 91% and 95% agreement at 473 nm and 632.8 nm, respectively.
    • Provided a detailed TiO2 phantom preparation protocol for reproducibility.

    Conclusions:

    • Established a route for phase-driven absorption assessment in turbid media.
    • Presented an initial framework for estimating µa from reconstructed phase data.
    • Laid the groundwork for future quantitative separation of absorption and scattering.