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Updated: Mar 19, 2026

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Blood Serum From Obese Women Raises ROS Production by Neural Stem Cells.

Phelipe Elias da Silva1, Natássia Caroline Resende Corrêa2, Natália Ferreira Silva3

  • 1Institute of Biomedical Sciences, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil.

Developmental Neurobiology
|March 18, 2026
PubMed
Summary
This summary is machine-generated.

Maternal obesity serum subtly increases oxidative stress in human neural stem cells (NSCs). While serum itself stresses NSCs, obese serum may amplify these effects, impacting early neurodevelopment.

Keywords:
cell differentiationcell viabilityembryonic stem cellsneural stem cellsobesityoxidative damage

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Maternal-Fetal Medicine

Background:

  • Maternal obesity is linked to adverse pregnancy outcomes and altered fetal development.
  • The direct impact of maternal serum factors on early human neural cells is not well understood.
  • Neural stem cells (NSCs) offer a model to study how metabolic and inflammatory changes affect neurodevelopment.

Purpose of the Study:

  • To investigate the direct effects of maternal serum from obese and non-obese women on human neural stem cells (NSCs).
  • To assess how maternal metabolic and inflammatory changes influence early neurodevelopmental processes in vitro.

Main Methods:

  • Human embryonic stem cells were differentiated into NSCs.
  • NSCs were exposed to serum from non-obese or obese women.
  • Evaluated cell viability, oxidative stress (ROS), metabolic activity, proliferation (Ki-67, PAX6), apoptosis markers (Caspase-3, p53), and progenitor markers.
  • Performed metabolomic profiling of maternal serum.

Main Results:

  • Serum exposure, regardless of maternal phenotype, induced stress responses in NSCs, including reduced viability and altered neural marker expression.
  • Distinct serum metabolomic signatures were observed between obese and non-obese donors, particularly in lipids and redox metabolites.
  • NSCs exposed to obese serum showed a modest but significant sustained increase in reactive oxygen species (ROS) and transient metabolic changes.
  • Obesity-related factors may subtly amplify oxidative stress responses in developing neural cells.

Conclusions:

  • In vitro serum exposure imposes significant stress on human neural stem cells.
  • Maternal obesity-associated factors may subtly exacerbate oxidative stress during early neurodevelopment.
  • The study highlights the limitations of in vitro models and the need for more physiologically relevant systems to study neurodevelopmental impacts.